Sex and B chromosome enigmas: model systems for the study of chromosome and genome evolution

• Grant number: 15/16661-1
• Support Opportunities: Research Projects - Thematic Grants
• Start date: May 01, 2017
• End date: April 30, 2023
• Field of knowledge: Biological Sciences - Genetics - Animal Genetics
• Principal Investigator: César Martins
• Grantee: César Martins
• Host Institution: Instituto de Biociências (IBB). Universidade Estadual Paulista (UNESP). Campus de Botucatu. Botucatu , SP, Brazil
• Associated researchers: Adauto Lima Cardoso ; Adriane Pinto Wasko ; Camila do Nascimento Moreira ; Danillo Pinhal ; David Ray ; Diogo Cavalcanti Cabral de Mello ; Flávia Karina Delella ; Guilherme Targino Valente ; Ivan Rodrigo Wolf ; Lígia Souza Lima Silveira da Mota ; Lucilene Delazari dos Santos ; Marcelo Ricardo Vicari ; Maria Dulcetti Vibranovski ; Michelle Orane Schemberger ; Ney Lemke ; Patricia Pasquali Parise Maltempi ; Rachel ONeill ; Renato Eugenio da Silva Diniz ; Silvia Mitiko Nishida ; Thomas David Kocher ; Vanessa Bellini Bardella ; Wagner Seixas da Silva
• Associated scholarship(s): 22/02544-7 - B chromosomes and epigenetic modifications: how DNA methylation relates to environmental stress in Astatotilapia latifasciata, BP.IC; 20/06188-5 - Molecular content, patterns of epigenetic changes and interference of supernumerary chromosomes in Drosophila melanogaster, BP.DR; 19/18190-7 - Towards a functional view of B chromosome biology and its interface with sex chromosomes, BP.PD; + associated scholarships 18/25350-8 - Development of genetic markers to sex identification of Astatotilapia latifasciata, BP.IC; 18/21772-5 - Cytogenomics of the treehoppers pest Aetalion reticulatum: analysis of repetitive DNAs and B chromosomes, BP.PD; 18/09553-6 - Genomic analysis of the rodent Holochilus sciureus (Cricetidae: Sigmodontinae: Oryzomyini) with emphasis on the interface between B and sex chromosomes, BP.PD; 18/08041-1 - What do you really know about genetics? An insight into the educational demands of high school students, BP.IC; 18/09127-7 - Effects of active genes on B chromosomes of Astatotilapia latifasciata and contributions in understanding the interaction between B and sex chromosomes, BP.IC; 18/09010-2 - Repetitive DNAs fraction analysis of Gryllus assimilis with emphasis on sex chromosomes, BP.IC; 17/25193-7 - PiRNA actuation in transposable elements silencing on B chromosome presence of the Astatotilapia latifasciata (Cichlidae), BP.DR; 17/07484-4 - Cell culture from Astatotilapia latifasciata and genome assembly of its B chromosome: contributions to the understanding of the effects of supernumerary chromosomes, BP.PD - associated scholarships

Abstract

Chromosomes are major components of cells, packing the genetic material in a perfect organized structural and functional pattern. However, the chromosomes behavior during cell cycle is subjected to genomic rearrangements producing a wide variety of functional consequences with impact in cell disorders and also generating evolutionary novelties. Although several factors that mediate genomic rearrangements are now known, there are still many unanswered questions. In the light of high throughput era in generation of biological data, chromosome studies have also advanced to a higher level exploring high scale analyses of DNA, RNA, and proteins, and their interactive networks. Therefore, this grant application intend to explore chromosomal polymorphism (associated to B chromosomes and sex chromosomes) as models to investigate the structural and functional nature of chromosomal rearrangements during evolution. In this way, the objective of this project is to analyze chromosome rearrangements in the light of massive molecular data obtained from different biological models, in order to advance in the identification of origin and fate of chromosome changes and to comprehend their evolutionary and disease consequences. Additionally, the project also aims to perform the scientific divulgation of chromosome facts, in the light of an evolutionary and function perspective, in order to enforce a dialog between science and society. (AU)

Scientific publications

(26)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

MARTINS, CESAR; JEHANGIR, MARYAM. A genomic glimpse of B chromosomes in cichlids. GENES & GENOMICS, v. 43, n. 3, SI, FEB 2021. (15/16661-1)

MILANI, DIOGO; RAMOS, ERICA; LORETO, VILMA; ANDREA MARTI, DARDO; CARDOSO, ADAUTO LIMA; MARTINEZ DE MORAES, KAREN CRISTIANE; MARTINS, CESAR; CABRAL-DE-MELLO, DIOGO CAVALCANTI. The satellite DNA AflaSAT-1 in the A and B chromosomes of the grasshopper Abracris flavolineata. BMC GENETICS, v. 18, AUG 29 2017. (15/05246-3, 11/19481-3, 15/16661-1)

COAN, RAFAEL L. B.; MARTINS, CESAR. Landscape of Transposable Elements Focusing on the B Chromosome of the Cichlid Fish Astatotilapia latifasciata. GENES, v. 9, n. 6, JUN 2018. (14/16763-6, 15/16661-1, 13/04533-3)

PALACIOS-GIMENEZ, OCTAVIO MANUEL; BARDELLA, VANESSA BELLINI; LEMOS, BERNARDO; CABRAL-DE-MELLO, DIOGO CAVALCANTI. Satellite DNAs are conserved and differentially transcribed among Gryllus cricket species. DNA Research, v. 25, n. 2, p. 137-147, APR 2018. (15/16661-1, 16/01506-3, 14/11763-8)

FERRETTI, ANA B. S. M.; MILANI, DIOGO; PALACIOS-GIMENEZ, OCTAVIO M.; RUIZ-RUANO, FRANCISCO J.; CABRAL-DE-MELLO, DIOGO C.. High dynamism for neo-sex chromosomes: satellite DNAs reveal complex evolution in a grasshopper. HEREDITY, v. 125, n. 3, p. 124-137, SEP 2020. (15/16661-1, 14/11763-8)

MILANI, DIOGO; RUIZ-RUANO, FRANCISCO J.; CAMACHO, JUAN PEDRO M.; CABRAL-DE-MELLO, DIOGO C.. Out of patterns, the euchromatic B chromosome of the grasshopper Abracris flavolineata is not enriched in high-copy repeats. HEREDITY, v. 127, n. 5, SEP 2021. (14/11763-8, 15/16661-1)

AZAMBUJA, MATHEUS; NOGAROTO, VIVIANE; MOREIRA-FILHO, ORLANDO; MARTINS, CESAR; SCHEMBERGER, MICHELLE ORANE; VICARI, MARCELO RICARDO. Major and minor U small nuclear RNAs genes characterization in a neotropical fish genome: Chromosomal remodeling and repeat units dispersion in Parodontidae. Gene, v. 826, p. 12-pg., 2022-06-05. (15/16661-1)

CARMELLO, BIANCA O.; COAN, RAFAEL L. B.; CARDOSO, ADAUTO L.; RAMOS, ERICA; FANTINATTI, BRUNO E. A.; MARQUES, DIEGO F.; OLIVEIRA, ROGERIO A.; VALENTE, GUILHERME T.; MARTINS, CESAR. The hnRNP Q-like gene is retroinserted into the B chromosomes of the cichlid fish Astatotilapia latifasciata. Chromosome Research, v. 25, n. 3-4, p. 277-290, OCT 2017. (11/03807-7, 15/16661-1, 13/04533-3)

MAHADEVARAJU, SHARVANI; FEAR, JUSTIN M.; AKEJU, MIRIAM; GALLETTA, BRIAN J.; PINHEIRO, MARA M. L. S.; AVELINO, CAMILA C.; CABRAL-DE-MELLO, DIOGO C.; CONLON, KATIE; DELL'ORSO, STAFANIA; DEMERE, ZELALEM; et al. Dynamic sex chromosome expression in Drosophila male germ cells. NATURE COMMUNICATIONS, v. 12, n. 1, FEB 9 2021. (15/20844-4, 17/14923-4, 17/26609-2, 15/16661-1, 13/08028-1, 19/15212-0)

CARDOSO, ADAUTO LIMA; VENTURELLI, NATALIA BORTHOLAZZI; DA CRUZ, IRENE; DE SA PATRONI, FABIO MALTA; DE MORAES, DIOGO; DE OLIVEIRA, ROGERIO ANTONIO; BENAVENTE, RICARDO; MARTINS, CESAR. Meiotic behavior, transmission and active genes of B chromosomes in the cichlid Astatotilapia latifasciata: new clues about nature, evolution and maintenance of accessory elements. Molecular Genetics and Genomics, v. 297, n. 4, p. 17-pg., 2022-06-15. (16/03912-9, 15/16661-1, 13/25234-4, 16/07743-7, 17/07484-4, 14/15756-6)

OLIVEIRA, JORDANA INACIO NASCIMENTO; CARDOSO, ADAUTO LIMA; WOLF, IVAN RODRIGO; DE OLIVEIRA, ROGERIO ANTONIO; MARTINS, CESAR. First characterization of PIWI-interacting RNA clusters in a cichlid fish with a B chromosome. BMC Biology, v. 20, n. 1, p. 18-pg., 2022-09-21. (15/16661-1, 16/09320-6, 17/25193-7)

MARTINS, CESAR; JEHANGIR, MARYAM. A genomic glimpse of B chromosomes in cichlids. GENES & GENOMICS, v. 43, n. 3, p. 10-pg., 2021-02-06. (15/16661-1)

CARDOSO, ADAUTO LIMA; DE ALMEIDA FANTINATTI, BRUNO EVARISTO; VENTURELLI, NATALIA BORTHOLAZZI; CARMELLO, BIANCA DE OLIVEIRA; DE OLIVEIRA, ROGERIO ANTONIO; MARTINS, CESAR. Epigenetic DNA Modifications Are Correlated With B Chromosomes and Sex in the Cichlid Astatotilapia latifasciata. FRONTIERS IN GENETICS, v. 10, APR 12 2019. (15/16661-1, 13/04533-3, 12/21546-9)

JEHANGIR, MARYAM; AHMAD, SYED F.; CARDOSO, ADAUTO L.; RAMOS, ERICA; VALENTE, GUILHERME T.; MARTINS, CESAR. De novo genome assembly of the cichlid fish Astatotilapia latifasciata reveals a higher level of genomic polymorphism and genes related to B chromosomes. CHROMOSOMA, v. 128, n. 2, p. 81-96, JUN 2019. (14/17683-6, 11/03807-7, 15/16661-1, 13/04533-3)

FERRETTI, ANA B. S. M.; RUIZ-RUANO, FRANCISCO J.; MILANI, DIOGO; LORETO, VILMA; MARTI, DARDO A.; RAMOS, ERICA; MARTINS, CESAR; CABRAL-DE-MELLO, DIOGO C.. How dynamic could be the 45S rDNA cistron? An intriguing variability in a grasshopper species revealed by integration of chromosomal and genomic data. CHROMOSOMA, v. 128, n. 2, p. 165-175, JUN 2019. (15/16661-1, 14/11763-8)

DULZ, THAIS APARECIDA; AZAMBUJA, MATHEUS; NASCIMENTO, VIVIANE DEMETRIO; LORSCHEIDER, CARLA ANDREA; NOLETO, RAFAEL BUENO; MOREIRA-FILHO, ORLANDO; NOGAROTO, VIVIANE; DINIZ, DEBORA; AFFONSO, PAULO ROBERTO ANTUNES DE MELLO; VICARI, MARCELO RICARDO. Karyotypic Diversification in TwoMegaleporinusSpecies (Characiformes, Anostomidae) Inferred fromIn SituLocalization of Repetitive DNA Sequences. ZEBRAFISH, v. 17, n. 5, p. 333-341, OCT 1 2020. (15/16661-1)

AHMAD, SYED F.; JEHANGIR, MARYAM; CARDOSO, ADAUTO L.; WOLF, IVAN R.; MARGARIDO, VLADIMIR P.; CABRAL-DE-MELLO, DIOGO C.; O'NEILL, RACHEL; VALENTE, GUILHERME T.; MARTINS, CESAR. B chromosomes of multiple species have intense evolutionary dynamics and accumulated genes related to important biological processes. BMC Genomics, v. 21, n. 1, SEP 23 2020. (18/03877-4, 15/16661-1, 14/16477-3)

AHMAD, SYED FARHAN; MARTINS, CESAR. The Modern View of B Chromosomes Under the Impact of High Scale Omics Analyses. CELLS, v. 8, n. 2, FEB 2019. (15/16661-1, 14/16477-3)

MILANI, DIOGO; BARDELLA, VANESSA B.; FERRETTI, ANA B. S. M.; PALACIOS-GIMENEZ, OCTAVIO M.; MELO, ADRIANA DE S.; MOURA, RITA C.; LORETO, VILMA; SONG, HOJUN; CABRAL-DE-MELLO, DIOGO C.. Satellite DNAs Unveil Clues about the Ancestry and Composition of B Chromosomes in Three Grasshopper Species. GENES, v. 9, n. 11, NOV 2018. (15/16661-1)

SCHEMBERGER, MICHELLE ORANE; NASCIMENTO, VIVIANE DEMETRIO; COAN, RAFAEL; RAMOS, ERICA; NOGAROTO, VIVIANE; ZIEMNICZAK, KALINE; VALENTE, GUILHERME TARGINO; MOREIRA-FILHO, ORLANDO; MARTINS, CESAR; VICARI, MARCELO RICARDO. DNA transposon invasion and microsatellite accumulation guide W chromosome differentiation in a Neotropical fish genome. CHROMOSOMA, v. 128, n. 4, p. 547-560, DEC 2019. (15/16661-1)

HOUBEN, ANDREAS; JONES, NEIL; MARTINS, CESAR; TRIFONOV, VLADIMIR. Evolution, Composition and Regulation of Supernumerary B Chromosomes. GENES, v. 10, n. 2, p. 4-pg., 2019-02-01. (15/16661-1)

PINHAL, DANILLO; BOVOLENTA, LUIZ A.; MOXON, SIMON; OLIVEIRA, ARTHUR C.; NACHTIGALL, PEDRO G.; ACENCIO, MARCIO L.; PATTON, JAMES G.; HILSDORF, ALEXANDRE W. S.; LEMKE, NEY; MARTINS, CESAR. Genome-wide microRNA screening in Nile tilapia reveals pervasive isomiRs' transcription, sex-biased arm switching and increasing complexity of expression throughout development. SCIENTIFIC REPORTS, v. 8, MAY 29 2018. (12/13450-1, 12/15589-7, 15/16661-1, 15/19176-7, 11/06465-0, 13/06864-7)

AHMAD, SYED FARHAN; JEHANGIR, MARYAM; SRIKULNATH, KORNSORN; MARTINS, CESAR. Fish genomics and its impact on fundamental and applied research of vertebrate biology. Reviews in Fish Biology and Fisheries, OCT 2021. (15/16661-1)

NASCIMENTO-OLIVEIRA, JORDANA INACIO; FANTINATTI, BRUNO EVARISTO ALMEIDA; WOLF, IVAN RODRIGO; CARDOSO, ADAUTO LIMA; RAMOS, ERICA; RIEDER, NATHALIE; DE OLIVEIRA, ROGERIO; MARTINS, CESAR. Differential expression of miRNAs in the presence of B chromosome in the cichlid fish Astatotilapia latifasciata. BMC Genomics, v. 22, n. 1, MAY 12 2021. (15/16661-1, 17/25193-7, 16/09320-6)

CARDOSO, ADAUTO LIMA; DE ALMEIDA FANTINATTI, BRUNO EVARISTO; VENTURELLI, NATALIA BORTHOLAZZI; CARMELLO, BIANCA DE OLIVEIRA; DE OLIVEIRA, ROGERIO ANTONIO; MARTINS, CESAR. Epigenetic DNA Modifications Are Correlated With B Chromosomes and Sex in the Cichlid Astatotilapia latifasciata. FRONTIERS IN GENETICS, v. 10, p. 14-pg., 2019-04-12. (12/21546-9, 13/04533-3, 15/16661-1)

MARTINS, CESAR; NAKAJIMA, RAFAEL T.; FANTINNATI, BRUNO E.; MARQUES, DIEGO F.; VENTURELLI, NATALIA B.; JEHANGIR, MARYAM; RAMOS, ERICA; OLIVEIRA, JORDANA; AHMAD, SYED F.; BOVOLENTA, LUIZ A.; et al. B chromosomes in the light of functional analyses. COMPARATIVE CYTOGENETICS, v. 12, n. 3, p. 2-pg., 2018-08-16. (15/16661-1, 13/04533-3)