Novel orthohepeviruses in wild rodents from Sao Paulo State, Brazil

Author(s)

de Souza, William Marciel ^{[1, 2]} ; Romeiro, Marilia Farignoli ^[2] ; Sabino-Santos, Jr., Gilberto ^[2] ; Motta Maia, Felipe Goncalves ^{[2, 3]} ; Fumagalli, Marcilio Jorge ^[2] ; Modha, Sejal ^[1] ; Teixeira Nunes, Marcio Roberto ^[4] ; Murcia, Pablo Ramiro ^[1] ; Moraes Figueiredo, Luiz Tadeu ^[2]

Affiliation

^[1] Univ Glasgow, MRC, Ctr Virus Res, Glasgow, Lanark - Scotland

^[2] Univ Sao Paulo, Ribeirao Preto Med Sch, Virol Res Ctr, Av Bandeirantes 3900, BR-14049900 Ribeirao Preto, SP - Brazil

^[3] Univ Sao Paulo, Lab Inst Biomed Sci, Sao Paulo - Brazil

^[4] Minist Hlth, Evandro Chagas Inst, Ctr Technol Innovat, Ananindeua, Para - Brazil

• Document type: Journal article
• Source: VIROLOGY; v. 519, p. 12-16, JUN 2018.
• Web of Science Citations: 7

Abstract

The Hepeviridae comprise single-stranded positive-sense RNA viruses classified into two genera, Orthohepevirus and Piscihepevirus. Orthohepeviruses have a wide host range that includes rodents, but previous studies had been restricted to rodents of the Muridae family. In this study, we applied a high-throughput sequencing approach to examine the presence of orthohepeviruses in rodents from Sao Paulo State, Brazil. We also used RT-PCR to determine the frequency of orthohepeviruses in our sampled population. We identified novel orthohepeviruses in blood samples derived from Necromys lasiurus (1.19%) and Calomys tener (3.66%). Therefore, our results expand the host range and viral diversity of the Hepeviridae family. (AU)

• FAPESP's process: 16/02568-2 - Research on hantavirus and other viruses in wild small mammals and their ectoparasites
• Grantee: Gilberto Sabino dos Santos Junior
• Support Opportunities: Scholarships in Brazil - Post-Doctoral
• FAPESP's process: 12/24150-9 - Research of virus in wild rodents, mosquitoes and ticks
• Grantee: William Marciel de Souza
• Support Opportunities: Scholarships in Brazil - Doctorate
• FAPESP's process: 17/13981-0 - Characterization, genomics and diagnostic of viruses with importance for public health in Brazil by high throughput sequencing
• Grantee: William Marciel de Souza
• Support Opportunities: Scholarships in Brazil - Post-Doctoral
• FAPESP's process: 16/01414-1 - Production of recombinant viral antigenic peptides and development of methods for serological diagnosis of infections by Chikungunya, Mayaro and Zika viruses
• Grantee: Marcilio Jorge Fumagalli
• Support Opportunities: Scholarships in Brazil - Master
• FAPESP's process: 14/20851-8 - Study about Alphavirus and Flavivirus infections in samples of blood bank
• Grantee: Marilia Farignoli Romeiro
• Support Opportunities: Scholarships in Brazil - Doctorate
• FAPESP's process: 15/05778-5 - Bioinformatics analysis of data of viral metagenomic of animals from Brazil and Antarctica
• Grantee: William Marciel de Souza
• Support Opportunities: Scholarships abroad - Research Internship - Doctorate
• FAPESP's process: 13/14929-1 - Detection of arbovirus and other RNA viruses by real time RT-PCR and study of the viroma
• Grantee: Luiz Tadeu Moraes Figueiredo
• Support Opportunities: Regular Research Grants
• FAPESP's process: 11/06810-9 - Detection and ecology of Hantavirus among wild small mammals and their ectoparasites
• Grantee: Gilberto Sabino dos Santos Junior
• Support Opportunities: Scholarships in Brazil - Doctorate
• FAPESP's process: 08/06411-4 - Detection of Hantavirus in rodents and study of dynamics of rodent-reservoir populations in the region of Ribeirão Preto.
• Grantee: Gilberto Sabino dos Santos Junior
• Support Opportunities: Scholarships in Brazil - Master
• FAPESP's process: 11/22663-6 - Study of the occurence of arboviroses in wild animals and mosquitoes in Brazilian biomes and agroecosystems
• Grantee: Felipe Gonçalves Motta Maia
• Support Opportunities: Scholarships in Brazil - Doctorate