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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Alteration of Surface Glycoproteins After Photodynamic Therapy

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Author(s):
de Faria Souza, Roberta Kelly [1] ; Silva Carvalho, Isabel Chaves [1] ; da Cunha Menezes Costa, Carolina Genuncio [1] ; da Silva, Newton Soares [2] ; Pacheco-Soares, Cristina [1]
Total Authors: 5
Affiliation:
[1] Univ Vale Paraiba UNIVAP, Inst Res & Dev IP&D, Lab Dynam Cellular Compartments, Ave Shishima Hifumi, 2911 Urbanova, BR-12244000 Sao Jose Dos Campos, SP - Brazil
[2] Univ Vale Paraiba UNIVAP, Inst Res & Dev IP&D, Lab Cell Biol & Tissue, Sao Jose Dos Campos, SP - Brazil
Total Affiliations: 2
Document type: Journal article
Source: Photomedicine and Laser Surgery; v. 36, n. 8, p. 452-456, AUG 2018.
Web of Science Citations: 1
Abstract

Background: Cell membranes have been identified as an important intracellular cancer treatment target, since the glycoconjugates present on the cell surface are involved in numerous cell functions. Photodynamic therapy (PDT) is a therapeutic modality employed in the treatment of tumors that uses visible light to activate a photosensitizer. Objective: This study analyzed the expression of surface carbohydrates after PDT with two different photosensitizers, 5-aminolevulinic acid (ALA) and Photosan-3. Methods: Mice were injected subcutaneously with 2x10(5) B16 cells. After 7-10 days, the presence of a tumor with a diameter of 3.6mm was observed. Photosan-3((R)) and 5-aminolevulinic acidALA were used in the PDT treatment. Control animals (not submitted to either laser treatment or photosensitizer injection) and treated animals were euthanized 15 days post-treatment. The tumors were irradiated with a red diode laser, =655nm, energy density of 10J.cm(-2), and power density of 45mW.cm(-2). After 2 weeks of treatment with PDT, the mice were euthanized, the tumors were collected, and the cell surfaces were labeled with lectins concanavalin A (ConA) and wheat germ agglutinin (WGA). Results: Fluorescence microscopy analysis of the cell surfaces with lectins ConA and WGA showed the presence of -mannose and -glucose. Conclusions: The combined effects of either Photosan-3 or ALA and red laser light on melanoma suggest an inhibitory glycosylation action from PDT on the surface of B16-F10 cells. (AU)

FAPESP's process: 11/05404-7 - Evaluation of the inactivation of breast cancer after photodynamic therapy in vivo
Grantee:Carolina Genúncio da Cunha Menezes Costa
Support type: Scholarships in Brazil - Master
FAPESP's process: 16/17984-1 - Evaluation glycans, heat shock proteins and fagocitose after photodynamic treatment.
Grantee:Cristina Pacheco Soares
Support type: Regular Research Grants
FAPESP's process: 09/15206-8 - Cellular mechanism of inactivating tumor by photodynamic therapy
Grantee:Cristina Pacheco Soares
Support type: Regular Research Grants
FAPESP's process: 11/05958-2 - Assessment genotoxicity of photodynamic therapy with phthalocyanine-chlorine-alumina (AlPc) and Alumina-chlorine-phthalocyanine liposomal (ALPcCL) in differenr cell lines.
Grantee:Andreza Cristina de Siqueira Silva
Support type: Scholarships in Brazil - Scientific Initiation