Alteration of Surface Glycoproteins After Photodynamic Therapy

Author(s)

de Faria Souza, Roberta Kelly ^[1] ; Silva Carvalho, Isabel Chaves ^[1] ; da Cunha Menezes Costa, Carolina Genuncio ^[1] ; da Silva, Newton Soares ^[2] ; Pacheco-Soares, Cristina ^[1]

Affiliation

^[1] Univ Vale Paraiba UNIVAP, Inst Res & Dev IP&D, Lab Dynam Cellular Compartments, Ave Shishima Hifumi, 2911 Urbanova, BR-12244000 Sao Jose Dos Campos, SP - Brazil

^[2] Univ Vale Paraiba UNIVAP, Inst Res & Dev IP&D, Lab Cell Biol & Tissue, Sao Jose Dos Campos, SP - Brazil

• Document type: Journal article
• Source: Photomedicine and Laser Surgery; v. 36, n. 8, p. 452-456, AUG 2018.
• Web of Science Citations: 1

Abstract

Background: Cell membranes have been identified as an important intracellular cancer treatment target, since the glycoconjugates present on the cell surface are involved in numerous cell functions. Photodynamic therapy (PDT) is a therapeutic modality employed in the treatment of tumors that uses visible light to activate a photosensitizer. Objective: This study analyzed the expression of surface carbohydrates after PDT with two different photosensitizers, 5-aminolevulinic acid (ALA) and Photosan-3. Methods: Mice were injected subcutaneously with 2x10(5) B16 cells. After 7-10 days, the presence of a tumor with a diameter of 3.6mm was observed. Photosan-3((R)) and 5-aminolevulinic acidALA were used in the PDT treatment. Control animals (not submitted to either laser treatment or photosensitizer injection) and treated animals were euthanized 15 days post-treatment. The tumors were irradiated with a red diode laser, =655nm, energy density of 10J.cm(-2), and power density of 45mW.cm(-2). After 2 weeks of treatment with PDT, the mice were euthanized, the tumors were collected, and the cell surfaces were labeled with lectins concanavalin A (ConA) and wheat germ agglutinin (WGA). Results: Fluorescence microscopy analysis of the cell surfaces with lectins ConA and WGA showed the presence of -mannose and -glucose. Conclusions: The combined effects of either Photosan-3 or ALA and red laser light on melanoma suggest an inhibitory glycosylation action from PDT on the surface of B16-F10 cells. (AU)

• FAPESP's process: 11/05404-7 - Evaluation of the inactivation of breast cancer after photodynamic therapy in vivo
• Grantee: Carolina Genúncio da Cunha Menezes Costa
• Support type: Scholarships in Brazil - Master
• FAPESP's process: 16/17984-1 - Evaluation glycans, heat shock proteins and fagocitose after photodynamic treatment.
• Grantee: Cristina Pacheco Soares
• Support type: Regular Research Grants
• FAPESP's process: 09/15206-8 - Cellular mechanism of inactivating tumor by photodynamic therapy
• Grantee: Cristina Pacheco Soares
• Support type: Regular Research Grants
• FAPESP's process: 11/05958-2 - Assessment genotoxicity of photodynamic therapy with phthalocyanine-chlorine-alumina (AlPc) and Alumina-chlorine-phthalocyanine liposomal (ALPcCL) in differenr cell lines.
• Grantee: Andreza Cristina de Siqueira Silva
• Support type: Scholarships in Brazil - Scientific Initiation