Advanced search
Start date
Betweenand
Related content
(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Glycaemic status affects the subgingival microbiome of diabetic patients

Full text
Author(s):
Longo, Priscila L. [1] ; Dabdoub, Shareef [2] ; Kumar, Purnima [2] ; Artese, Hilana P. C. [3] ; Dib, Sergio A. [4] ; Romito, Giuseppe A. [3] ; Alves Mayer, Marcia Pinto [1, 3]
Total Authors: 7
Affiliation:
[1] Univ Sao Paulo, Inst Biomed Sci, Dept Microbiol, Av Prof Lineu Prestes 1374, BR-05508900 Sao Paulo, SP - Brazil
[2] Ohio State Univ, Coll Dent, Div Periodontol, Columbus, OH 43210 - USA
[3] Univ Sao Paulo, Sch Dent, Dept Stomatol, Div Periodont, Sao Paulo, SP - Brazil
[4] Univ Fed Sao Paulo, Sch Med, Dept Endocrinol, Sao Paulo, SP - Brazil
Total Affiliations: 4
Document type: Journal article
Source: JOURNAL OF CLINICAL PERIODONTOLOGY; v. 45, n. 8, p. 932-940, AUG 2018.
Web of Science Citations: 4
Abstract

AimPeriodontitis iscorrelated with type 2 diabetes mellitus (T2DM), but little is known about glycaemic status effect on subgingival microbiota associated withperiodontitis. This study evaluated if periodontal microbiome of T2DM patients is affected by glycaemic status. Materials and methodsTwenty-one T2DM non-smoking patients with chronic periodontitis and body mass index 40kg/m(2) were allocated into two groups according to systemic glycaemic status: inadequate (DMI- HbA1c8%) and adequate (DMA- HbA1c <7.8%). Subgingival biofilm was collected from sites with moderate (PD=4-6mm) and severe disease (PD7mm) in two quadrants. The V5-V6 hypervariable region of the 16SrRNA was sequenced using the GS-FLX-454 Titanium platform. Sequences were compared with HOMD database using QIIME and PhyloToAST pipelines. Statistical comparisons were made using two-sample t-tests. ResultsDMA microbiome presented higher diversity than DMI. Inadequate glycaemic control favoured fermenting species, especially those associated with propionate/succinate production, whereas those forming butyrate/pyruvate was decreased in DMI. Higher abundances of anginosus group and Streptococcus agalactiae in DMI may indicate that subgingival sites can be reservoir of potentially invasive pathogens. Altered subgingival microbiome in DMI may represent an additional challenge in the periodontal treatment of these patients and in the prevention of more invasive infections. ConclusionGlycaemic status in T2DM patients seems to modulate subgingival biofilm composition. (AU)

FAPESP's process: 13/20537-9 - Analysis of data from 16SrRNA sequencing generated by high throughput of subgingival biofilm samples from diabetic patients
Grantee:Priscila Larcher Longo
Support Opportunities: Scholarships abroad - Research Internship - Post-doctor
FAPESP's process: 11/10057-4 - Analysis of subgingival microbiota diversity and serum cytokine and gingival fluid profile related to glycemic control in subjects with diabetes mellitus type II
Grantee:Priscila Larcher Longo
Support Opportunities: Scholarships in Brazil - Post-Doctoral
FAPESP's process: 11/18618-5 - Subgingival microbiota diversity and serum and gingival fluid cytokines profile related to glycemic control in subjects with Type II Diabetes
Grantee:Marcia Pinto Alves Mayer
Support Opportunities: Regular Research Grants