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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

EVALUATION OF ENEMAS CONTAINING SUCRALFATE IN TISSUE CONTENT OF MUC-2 PROTEIN IN EXPERIMENTAL MODEL OF DIVERSION COLITIS

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Author(s):
Araya Fernandez, Oscar Orlando [1] ; Pereira, Jose Aires [1] ; Campos, Fabio Guilherme [1] ; Araya, Carolina Mardegan [1] ; Marinho, Gabriele Escocia [1] ; Novo, Rafaela de Souza [1] ; de Oliveira, Thais Silva [1] ; Franceschi, Yara Tinoco [1] ; Real Martinez, Carlos Augusto [1]
Total Authors: 9
Affiliation:
[1] Univ Sao Francisco, Programa Posgrad Ciencias, Lab Invest Med, Braganca Paulista, SP - Brazil
Total Affiliations: 1
Document type: Journal article
Source: ABCD-ARQUIVOS BRASILEIROS DE CIRURGIA DIGESTIVA-BRAZILIAN ARCHIVES OF DIGESTIVE SURGERY; v. 31, n. 3 2018.
Web of Science Citations: 3
Abstract

Background: The effects of topical application of sucralfate (SCF) on the tissue content of MUC-2 protein have not yet been evaluated in experimental models of diversion colitis. Aim: To measure the tissue content of MUC-2 protein in the colonic mucosa diverted from fecal stream submitted to the SCF intervention. Methods: Thirty-six rats underwent derivation of intestinal transit through proximal colostomy and distal mucous fistula. The animals were divided into three groups which were submitted application of enemas with saline, SCF 1 g/kg/day and SCF 2 g/kg/day. Each group was divided into two subgroups, according to euthanasia was done after two or four weeks. The colitis diagnosis was established by histopathological study and the inflammatory intensity was evaluated by previously validated scale. The MUC-2 protein was identified by immunohistochemistry and the tissue content was measured computerized morphometry). Results: The application of enemas with SCF in the concentration of 2 g/kg/day reduced inflammatory score of the segments that were diverted from fecal stream. The content of MUC-2 in diverted colon of the animals submitted to the intervention with SCF, independently of intervention period and the used concentration, was significantly greater than animals submitted to the application of enemas containing saline (p< 0.01). The content of MUC-2 after the intervention with SCF in the concentration of 2 g/kg/day was significantly higher when compared to the animals submitted to the application containing SCF at concentration of 1.0 g/kg/day (p<0.01). The tissue content of MUC-2 reached the highest values after intervention with SCF in the concentration of 2 g/kg/day for four weeks (p<0.01). Conclusion: The preventive application of enemas containing SCF reduces the inflammatory score and avoids the reduction of tissue content of MUC-2, suggesting that the substance is a valid therapeutic strategy to preserve the mucus layer that covers the intestinal epithelium. (AU)

FAPESP's process: 10/12492-7 - Evaluation of antioxidant effects of 5-aminosalycylic acid, N-acetylcysteine, sucralfate, curcumin and aqueous extract of Ilex paraguariensis in contente and pattern of tissue expression of intercellular junctions proteins: study in rats
Grantee:Carlos Augusto Real Martinez
Support Opportunities: Regular Research Grants