Komagataeibacter rhaeticus grown in sugarcane molasses-supplemented culture medium as a strategy for enhancing bacterial cellulose production

Author(s):Show less -

Machado, Rachel T. A. ^[1] ; Meneguin, Andrea Bagliotti ^{[2, 1]} ; Sabio, Rafael Miguel ^[1] ; Franco, Douglas Faza ^[3] ; Antonio, Selma G. ^[3] ; Gutierrez, Junkal ^{[4, 5]} ; Tercjak, Agnieszka ^[4] ; Berretta, Andresa A. ^[6] ; Ribeiro, Sidney J. L. ^[3] ; Lazarini, Silmara C. ^[1] ; Lustri, Wilton R. ^[1] ; Barud, Hernane S. ^[1]

Affiliation

^[1] Univ Araraquara, UNIARA, BR-14801320 Araraquara, SP - Brazil

^[2] Univ Fed Piaui UFPI, Ctr Ciencias Nat CNN, Interdisciplinary Lab Adv Mat, BR-64049550 Teresina, PI - Brazil

^[3] Sao Paulo State Univ UNESP, Inst Chem, BR-14800060 Araraquara, SP - Brazil

^[4] Univ Basque Country, UPV EHU, Fac Engn, Grp Mat Technol GMT, Dept Chem & Environm Engn, Plaza Europa 1, Donostia San Sebastian 20018 - Spain

^[5] Univ Basque Country, UPV EHU, Fac Engn Vitoria Gasteiz, C Nieves Cano 12, Vitoria 01006 - Spain

^[6] Apis Flora Ind Coml Ltda, Lab Pesquisa Desenvolvimento & Inovacao, BR-14020670 Ribeirao Preto, SP - Brazil

• Document type: Journal article
• Source: INDUSTRIAL CROPS AND PRODUCTS; v. 122, p. 637-646, OCT 15 2018.
• Web of Science Citations: 5

Abstract

Komagataeibacter rhaeticus, a bacterium isolated from Kombucha tea, was used to produce bacterial cellulose (BC) through its cultivation in a static sugarcane molasses (SCM) supplemented-culture medium (totally or partially), as an alternative carbon source. BC membranes were characterized by different physicochemical analysis using Fourier transform infrared spectroscopy (FTIR), X-ray diffraction (XRD), field emission gun scanning electron microscopy (FEG-SEM), thermogravimetry analysis (TGA) and PeakForce quantitative nano mechanics atomic force microscopy (PeakForce (QNM-AFM)). FTIR, XRD and TGA results suggest great similarity among all membranes produced by distinct culture media. Although the glucose (F1) and SCM (F6) media presented the lowest BC yield, all SCM-supplemented culture media (from F2 to F5) showed BC yield values similar to the HS culture medium (F0). FEG-SEM analysis showed that as higher SCM concentrations on culture media higher dense nanofibers network could be prepared. Quantitative nanomechanical results obtained by AFM technique corroborate FEG-SEM analysis besides show smoother and more flexible BC membranes as a function of the increasing of the SCM concentrations. The modification of the carbon source of the culture medium with an important by-product of Brazilian agroindustry appears as a viable alternative to reduce cost of BC production (of up to 20.06%) besides increase the possibilities of industrial scale BC preparation. (AU)