Neurotoxic lesions of the pedunculopontine tegmental nucleus impair the elaboration of postictal antinociception

Author(s)

de Oliveira, Rithiele Cristina ^[1] ; de Oliveira, Ricardo ^{[1, 2, 3]} ; Falconi-Sobrinho, Luiz Luciano ^{[1, 3]} ; Biagioni, Audrey Franceschi ^{[1, 4]} ; Almada, Rafael Carvalho ^{[1, 3]} ; dos Anjos-Garcia, Tayllon ^[1] ; Bazaglia-de-Sousa, Guilherme ^[1] ; Khan, Asmat Ullah ^{[1, 5]} ; Coimbra, Norberto Cysne ^{[1, 3, 6]}

Affiliation

^[1] Univ Sao Paulo, Ribeirao Preto Med Sch, FMRP, Lab Neuroanat & Neuropsychobiol, Dept Pharmacol, Av Bandeirantes 3900, BR-14049900 Ribeirao Preto, SP - Brazil

^[2] Univ Fed Mato Grosso, Med Sch UFMT, Av Alexandre Ferronato 1200, Reserva 35, BR-78550000 Sinop, Mato Grosso - Brazil

^[3] Behav Neurosci Inst INeC, Ave Cafe 2450, BR-14220030 Ribeirao Preto, SP - Brazil

^[4] Scuola Super Avanzati Trieste SISSA, Via Bonomea 265, I-34136 Trieste, TS - Italy

^[5] Univ Poonch Rawalakot, Sch Med & Hlth Sci, Dept Eastern Med & Surg, Hajira Rd, Rawalakot 12350, Azad Jammu & Ka - Pakistan

^[6] Univ Sao Paulo, Multiuser Ctr Neuroelectrophysiol, Ribeirao Preto Med Sch, Dept Anat & Surg, FMRP, Av Bandeirantes 3900, BR-14049900 Ribeirao Preto, SP - Brazil

• Document type: Journal article
• Source: Physiology & Behavior; v. 194, p. 162-169, OCT 1 2018.
• Web of Science Citations: 1

Abstract

Generalised tonic-clonic seizures, generated by abnormal neuronal hyper-activity, cause a significant and long-lasting increase in the nociceptive threshold. The pedunculopontine tegmental nucleus (PPTN) plays a crucial role in the regulation of seizures as well as the modulation of pain, but its role in postictal antinociceptive processes remains unclear. In the present study, we aimed to investigate the involvement of PPTN neurons in the postictal antinociception. Wistar rats had their tail-flick baseline recorded and were injected with ibotenic acid (1.0 mu g/0.2 mu L) into the PPTN, aiming to promote a local neurotoxic lesion. Five days after the neuronal damage, pentylenetetrazole (PTZ; 64 mg/kg) was intraperitoneally administered to induce tonic-clonic seizures. The tail withdrawal latency was measured immediately after the seizures (0 min) and subsequently at 10-min intervals until 130 min after the seizures were induced pharmacologically. Ibotenic acid microinjected into the PPTN did not reduce the PTZ-induced seizure duration and severity, but it diminished the postictal antinociception from 0 to 130 min after the end of the PTZ-induced tonic-clonic seizures. These results suggest that the postictal antinociception depends on the PPTN neuronal cells integrity. (AU)

• FAPESP's process: 11/09850-1 - study of serotoninergic pathways that connect the dorsal raphe nucleus to the Lateral Septal Area on the Modulation of Defensive Behavior Organized by periaqueductal gray performed by the prefrontal cortex
• Grantee: Ricardo de Oliveira
• Support Opportunities: Scholarships in Brazil - Post-Doctoral
• FAPESP's process: 14/10742-7 - Paresthesia as a new approach of study for panic disorder: basic and clinical research
• Grantee: Audrey Franceschi Biagioni
• Support Opportunities: Scholarships in Brazil - Post-Doctoral
• FAPESP's process: 08/08955-1 - Involvement of the Dorsal Raphe Nucleus Neural Networks and Serotonergic Neurotransmittion in the Locus Coeruleus in Antinociceptive Processess Induced by Oriented Escape Reaction Evoked by Chemical Stimulation of the Medial Hypothalamus.
• Grantee: Audrey Franceschi Biagioni
• Support Opportunities: Scholarships in Brazil - Master
• FAPESP's process: 09/00668-6 - Study of the involvement of cholinergic pathways form tegmentar pedunculopontine nucleus to monoaminergic nuclei of the pain endogenous inhibitory system in the post-ictal antinociception
• Grantee: Norberto Cysne Coimbra
• Support Opportunities: Regular Research Grants
• FAPESP's process: 13/10984-8 - Influence of the anterior cingulate cortex and involvement of hypothalamic NMDA and AMPA/kainite glutamatergic receptorsin the organization of the defensive behaviorand innate fear-induced antinociception elaborated by posterior hypothalamus
• Grantee: Luiz Luciano Falconi Sobrinho
• Support Opportunities: Scholarships in Brazil - Master
• FAPESP's process: 12/22681-7 - Involvement of Opioid and Endocanabinoid Receptors of the Substantia Nigra, Pars Reticulata, in the Modulation of the Activity of Nigro-Tectal GABAergic Pathways during the Organization of Defensive Behaviour of Mice Confronted with Venomous Snakes
• Grantee: Rafael Carvalho Almada
• Support Opportunities: Scholarships in Brazil - Post-Doctoral