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Study of serotoninergic pathways that connect the dorsal raphe nucleus to the lateral septal area on the modulation of defensive behavior organized by periaqueductal gray performed by the prefrontal cortex

Grant number: 11/09850-1
Support type:Scholarships in Brazil - Post-Doctorate
Effective date (Start): December 01, 2011
Effective date (End): February 28, 2014
Field of knowledge:Biological Sciences - Pharmacology
Principal Investigator:Norberto Cysne Coimbra
Grantee:Ricardo de Oliveira
Home Institution: Faculdade de Medicina de Ribeirão Preto (FMRP). Universidade de São Paulo (USP). Ribeirão Preto , SP, Brazil

Abstract

Electrical stimulation and chemical dorsolateral periaqueductal gray (SCPdl), elicits defensive responses associated with fear. The lateral septal area (ASL) receives serotonergic afferents from the dorsal raphe nucleus (DRN) and sends efferent projections to the periaqueductal gray. The purpose of this study is to investigate the modulatory mechanism of serotoninergic projections from the DRN and intended to ASL, the activity of pathways from the ASL, which extend to SCPdl, determining the influence of such pathways on behavior. For this, we will study the effects of pharmacological intervention in the activity of these pathways through microinjections of S-14506 (agonist of 5-HT1A), (S)-WAY 100135 (antagonist of 5-HT1A), DOI (5-HT2A/2C receptor agonist) and RS-102221 (antagonist of 5-HT2C receptors) in ASL, and are recorded after microinjections of drugs, the behavioral responses elicited by chemical stimulation, with local administration of glutamate, SCPdl. In an independent group of animals will be microinjected 5-7-di-hydroxytryptamine, a neurotoxin specific for serotonergic neurons in DRN and, shortly there after, and recorded the behavioral responses elicited by chemical stimulation of SCPdl. To verify the functional neuroanatomy of connective ASL with the NDR and will SCPdl microinjected neurotraçador the bidirectional neurotracer, biodextran (BDA-3000), revealed with DAB, followed by an immunohistochemical reaction for tryptophan hydroxylase, in order to investigate the precise location from which the DRN serotoninergic connections to ASL, the latter reaction process revealed by VIP-DAB.

Scientific publications (5)
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
DE OLIVEIRA, RITHIELE CRISTINA; DE OLIVEIRA, RICARDO; FALCONI-SOBRINHO, LUIZ LUCIANO; BIAGIONI, AUDREY FRANCESCHI; ALMADA, RAFAEL CARVALHO; DOS ANJOS-GARCIA, TAYLLON; BAZAGLIA-DE-SOUSA, GUILHERME; KHAN, ASMAT ULLAH; COIMBRA, NORBERTO CYSNE. Neurotoxic lesions of the pedunculopontine tegmental nucleus impair the elaboration of postictal antinociception. Physiology & Behavior, v. 194, p. 162-169, OCT 1 2018. Web of Science Citations: 1.
ULLAH, FARHAD; DOS ANJOS-GARCIA, TAYLLON; MENDES-GOMES, JOYCE; HIBRAHIM ELIAS-FILHO, DAOUD; FALCONI-SOBRINHO, LUIZ LUCIANO; DE FREITAS, RENATO LEONARDO; KHAN, ASMAT ULLAH; DE OLIVEIRA, RICARDO; COIMBRA, NORBERTO CYSNE. Connexions between the dorsomedial division of the ventromedial hypothalamus and the dorsal periaqueductal grey matter are critical in the elaboration of hypothalamically mediated panic-like behaviour. Behavioural Brain Research, v. 319, p. 135-147, FEB 15 2017. Web of Science Citations: 12.
DE OLIVEIRA, RICARDO; DE OLIVEIRA, RITHIELE CRISTINA; FALCONI-SOBRINHO, LUIZ LUCIANO; SOARES, JR., RAIMUNDO DA SILVA; COIMBRA, NORBERTO CYSNE. 5-Hydroxytryptamine(2A/2C) receptors of nucleus raphe magnus and gigantocellularis/paragigantocellularis pars alpha reticular nuclei modulate the unconditioned fear-induced antinociception evoked by electrical stimulation of deep layers of the superior colliculus and dorsal periaqueductal grey matter. Behavioural Brain Research, v. 316, p. 294-304, JAN 1 2017. Web of Science Citations: 9.
DE FREITAS, RENATO LEONARDO; MEDEIROS, PRISCILA; DA SILVA, JULIANA ALMEIDA; DE OLIVEIRA, RITHIELE CRISTINA; DE OLIVEIRA, RICARDO; ULLAH, FARHAD; KHAN, ASMAT ULLAH; COIMBRA, NORBERTO CYSNE. THE mu(1)-OPIOID RECEPTOR AND 5-HT2A- AND 5HT(2C)-SEROTONERGIC RECEPTORS OF THE LOCUS COERULEUS ARE CRITICAL IN ELABORATING HYPOALGESIA INDUCED BY TONIC AND TONIC-CLONIC SEIZURES. Neuroscience, v. 336, p. 133-145, NOV 12 2016. Web of Science Citations: 5.
BIAGIONI, AUDREY FRANCESCHI; DE OLIVEIRA, RITHIELE CRISTINA; DE OLIVEIRA, RICARDO; DA SILVA, JULIANA ALMEIDA; DOS ANJOS-GARCIA, TAYLION; RONCON, CAMILA MARRONI; CORRADO, ALEXANDRE PINTO; ZANGROSSI, JR., HELIO; COIMBRA, NORBERTO CYSNE. 5-Hydroxytryptamine(1A) receptors in the dorsomedial hypothalamus connected to dorsal raphe nucleus inputs modulate defensive behaviours and mediate innate fear-induced antinociception. European Neuropsychopharmacology, v. 26, n. 3, p. 532-545, MAR 2016. Web of Science Citations: 17.

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