|Support type:||Scholarships in Brazil - Post-Doctorate|
|Effective date (Start):||December 01, 2011|
|Effective date (End):||February 28, 2014|
|Field of knowledge:||Biological Sciences - Pharmacology - Neuropsychopharmacology|
|Principal researcher:||Norberto Cysne Coimbra|
|Grantee:||Ricardo de Oliveira|
|Home Institution:||Faculdade de Medicina de Ribeirão Preto (FMRP). Universidade de São Paulo (USP). Ribeirão Preto , SP, Brazil|
Electrical stimulation and chemical dorsolateral periaqueductal gray (SCPdl), elicits defensive responses associated with fear. The lateral septal area (ASL) receives serotonergic afferents from the dorsal raphe nucleus (DRN) and sends efferent projections to the periaqueductal gray. The purpose of this study is to investigate the modulatory mechanism of serotoninergic projections from the DRN and intended to ASL, the activity of pathways from the ASL, which extend to SCPdl, determining the influence of such pathways on behavior. For this, we will study the effects of pharmacological intervention in the activity of these pathways through microinjections of S-14506 (agonist of 5-HT1A), (S)-WAY 100135 (antagonist of 5-HT1A), DOI (5-HT2A/2C receptor agonist) and RS-102221 (antagonist of 5-HT2C receptors) in ASL, and are recorded after microinjections of drugs, the behavioral responses elicited by chemical stimulation, with local administration of glutamate, SCPdl. In an independent group of animals will be microinjected 5-7-di-hydroxytryptamine, a neurotoxin specific for serotonergic neurons in DRN and, shortly there after, and recorded the behavioral responses elicited by chemical stimulation of SCPdl. To verify the functional neuroanatomy of connective ASL with the NDR and will SCPdl microinjected neurotraçador the bidirectional neurotracer, biodextran (BDA-3000), revealed with DAB, followed by an immunohistochemical reaction for tryptophan hydroxylase, in order to investigate the precise location from which the DRN serotoninergic connections to ASL, the latter reaction process revealed by VIP-DAB.