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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Characteristics of the Epithelial-Mesenchymal Transition in Primary and Paired Metastatic Canine Mammary Carcinomas

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Author(s):
Raposo-Ferreira, Talita M. M. [1] ; Brisson, Becky K. [2] ; Durham, Amy C. [3, 4] ; Laufer-Amorim, Renee [5] ; Kristiansen, Veronica [6] ; Pure, Ellen [4, 7] ; Volk, Susan W. [4, 2, 7] ; Sorenmo, Karin [4, 7]
Total Authors: 8
Affiliation:
[1] Sao Paulo State Univ, UNESP, Sch Agr & Veterinarian Sci, Dept Vet Clin & Surg, Jaboticabal - Brazil
[2] Univ Penn, Sch Vet Med, Dept Clin Sci & Adv Med, Philadelphia, PA 19104 - USA
[3] Univ Penn, Sch Vet Med, Dept Pathobiol, Philadelphia, PA 19104 - USA
[4] Univ Penn, Penn Vet Canc Ctr, Philadelphia, PA 19104 - USA
[5] Sao Paulo State Univ, Sch Vet Med & Anim Sci, Dept Vet Clin, UNESP, Botucatu, SP - Brazil
[6] Norwegian Univ Life Sci, Fac Vet Med & Biosci, Dept Compan Anim Clin Sci, Oslo - Norway
[7] Univ Penn, Sch Vet Med, Dept Biomed Sci, Philadelphia, PA 19104 - USA
Total Affiliations: 7
Document type: Journal article
Source: VETERINARY PATHOLOGY; v. 55, n. 5, p. 622-633, SEP 2018.
Web of Science Citations: 5
Abstract

The epithelial-mesenchymal transition (EMT) is a dynamic process linked to metastasis in many tumor types, including mammary tumors. In this study, we evaluated E-cadherin and vimentin immunolocalization in primary canine mammary carcinomas (20 cases) and their respective metastases, as well as their relationship with the core regulators SNAIL/SLUG. To assess the number of cells undergoing the process of EMT, we quantitated double-positive (E-cadherin(+)/vinnentin(+)) cells using immunofluorescence, via cell counting and image analysis. In addition, SNAIL/SLUG expression was evaluated by established immunohistochemical methods. Primary tumors had significantly more E-cadherin(+)/vinnentin(+) co-expression than their paired respective lymph node or distant metastasis, respectively. Furthermore, the percentage of E-cadherin(+)/vinnentin(+) cells in grade II and III carcinomas was significantly higher than in grade I tumors. Primary tumors had significantly higher SNAIL/SLUG expression when analyzed based on the percentage of positive cells compared with their respective distant metastases in pairwise comparisons. An inverse correlation was noted between SNAIL/SLUG immunoreactivity and percentage of E-cadherin(+)/vinnentin(+) immunopositive cells in primary tumor samples when SNAIL/SLUG immunoreactivity was grouped into 2 categories (high versus low) based on percentage-positive staining. These results show a positive correlation between E-cadherin(+)/vinnentin(+) cells and higher tumor grade, establish differences between primary tumor and their respective metastases, and provide further support that EMT plays a critical role in the metastasis of canine mammary carcinoma. Furthermore, these data suggest that modulation of this process could provide greater therapeutic control and provide support for further research to determine if E-cadherin(+)/vinnentin(+) co-immunoreactivity imparts predictive value in the clinical outcome of patients with canine mammary carcinomas. (AU)

FAPESP's process: 15/09046-9 - Evaluation of prognostic factors and therapeutic targets related to epithelial-mesenchymal transition and tumor microenvironment of canine mammary carcinomas
Grantee:Talita Mariana Morata Raposo Ferreira
Support Opportunities: Scholarships abroad - Research Internship - Doctorate