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Study on large-scale of gene expression of bitches mammary carcinomas

Grant number: 13/03940-4
Support type:Scholarships in Brazil - Doctorate
Effective date (Start): June 01, 2013
Effective date (End): August 31, 2016
Field of knowledge:Agronomical Sciences - Veterinary Medicine
Principal Investigator:Renee Laufer Amorim
Grantee:Talita Mariana Morata Raposo Ferreira
Home Institution: Faculdade de Ciências Agrárias e Veterinárias (FCAV). Universidade Estadual Paulista (UNESP). Campus de Jaboticabal. Jaboticabal , SP, Brazil
Associated scholarship(s):15/09046-9 - Evaluation of prognostic factors and therapeutic targets related to epithelial-mesenchymal transition and tumor microenvironment of canine mammary carcinomas, BE.EP.DR

Abstract

Canine mammary tumor is the most common tumor in bitches, as in women and their biological and molecular behavior resembles what occurs in women,so bitches can be an excellent comparative model for understanding the carcinogenic process of this neoplasm. Metastasis is a common consequence and the leading cause of mortality from this disease in both species. The most common histological type of bitches carcinoma is carcinoma in mixed tumor. This is an uncommon tumor in women, making the dog a good model to study this type of neoplasia. Moreover, the frequency of metastasis of this histological type is low when compared to the simple carcinomas, interesting fact, since carcinomas in mixed tumor phenotype have an epithelial mesenchymal transition (EMT) that knowingly facilitates and supports the metastatic process. Faced with this fact, we propose to identify genes differentially expressed in simple and mixed tumor carcinomas of bitches, using the technique of gene expression on a large scale, array platforms, and thus recognize the molecular mechanisms involved in the processes of EMT and metastatic potential. The results will be validated by RT-qPCR and immunohistochemistry method. The data will also be compared with clinical and histopathological parameters.