| Full text | |
| Author(s): |
Milanez, Guilherme P.
[1]
;
Werle, Catierine H.
[1]
;
Amorim, Mariene R.
[1]
;
Ribeiro, Rafael A.
[1]
;
Tibo, Luiz H. S.
[1]
;
Roque-Barreira, Maria Cristina
[2]
;
Oliveira, Aline F.
[2]
;
Brocchi, Marcelo
[1]
Total Authors: 8
|
| Affiliation: | [1] Univ Estadual Campinas, Inst Biol, Dept Genet Evolut Microbiol & Immunol, Campinas, SP - Brazil
[2] Univ Sao Paulo, Dept Cellular & Mol Biol, Fac Med Ribeirao Preto, Sao Paulo - Brazil
Total Affiliations: 2
|
| Document type: | Journal article |
| Source: | FRONTIERS IN MICROBIOLOGY; v. 9, AUG 22 2018. |
| Web of Science Citations: | 0 |
| Abstract | |
Salmonella enterica infection is a major public health concern worldwide, particularly when associated with other medical conditions. The serovars Typhimurium and Enteritidis are frequently associated with an invasive illness that primarily affects immunocompromised adults and children with HIV, malaria, or malnutrition. These serovars can also cause infections in a variety of animal hosts, and they are the most common isolates in poultry materials. Here, we described S. Enteritidis mutants, where hupA and hupB genes were deleted, and evaluated their potential use as liveattenuated vaccine candidates. In vitro, the mutants behaved like S. Typhimurium described previously, but there were some particularities in macrophage invasion and survival experiments. The virulence and immunogenicity of the mutant lacking both hupA and hupB (PT4 Delta hupAB) were evaluated in a BALB/c mice model. This mutant was highly attenuated and could, therefore, be administrated at doses higher than 10(9) CFU/treatment, which was sufficient to protect all treated mice challenged with the wild-type parental strain with a single dose. Additionally, the PT4 Delta hupAB strain induced production of specific IgG and IgA antibodies against Salmonella and TH1-related cytokines (IFN-gamma and TNE-alpha), indicating that this strain can induce systemic and mucosal protection in the murine model. Additional studies are needed to better understand the mechanisms that lead to attenuation of the double-mutant PT4 Delta hupAB and to elucidate the immune response induced by immunization using this strain. However, our data allow us to state that hupAB mutants could be potential candidates to be explore as live-attenuated vaccines. (AU) | |
| FAPESP's process: | 13/04088-0 - Lectin from Pathogens |
| Grantee: | Maria Cristina Roque Antunes Barreira |
| Support Opportunities: | Research Projects - Thematic Grants |
| FAPESP's process: | 08/57354-0 - Avaliacao do potencial imunogenico e protetor de novas linhagens atenuadas de salmonella enterica typhimurium |
| Grantee: | Aline Ferreira de Oliveira Pereira |
| Support Opportunities: | Scholarships in Brazil - Post-Doctoral |
| FAPESP's process: | 12/05382-6 - Virulence evaluation and Differential gene expression in vivo of Salmonella enterica mutants for nucleoid associated proteins |
| Grantee: | Guilherme Paier Milanez |
| Support Opportunities: | Scholarships in Brazil - Doctorate |
| FAPESP's process: | 14/13412-8 - Salmonella enterica: Roles of Naps in pathogenicity and virulence. |
| Grantee: | Marcelo Brocchi |
| Support Opportunities: | Regular Research Grants |
| FAPESP's process: | 14/05359-0 - Distribuição de paracoccina na superfície de Paracoccidioides brasiliensis e interação com receptores de células da imunidade inata: papel na fase aguda da paracoccidioidomicose. |
| Grantee: | Aline Ferreira de Oliveira Pereira |
| Support Opportunities: | Scholarships in Brazil - Post-Doctoral |