Automated dispersive liquid-liquid microextraction based on the solidification of the organic phase

Author(s)

Vargas Medina, Deyber Arley ^{[1, 2]} ; Santos-Neto, Alvaro Jose ^[1] ; Cerda, Victor ^[2] ; Maya, Fernando ^[2]

Affiliation

^[1] Univ Sao Paulo, Sao Carlos Inst Chem, BR-13566590 Sao Carlos, SP - Brazil

^[2] Univ Balearic Isl, Dept Chem, E-07122 Palma De Mallorca - Spain

• Document type: Journal article
• Source: Talanta; v. 189, p. 241-248, NOV 1 2018.
• Web of Science Citations: 6

Abstract

In this work, the dispersive liquid-liquid microextraction technique based on the solidification of the organic phase (DLLME-SFO) has been automated for the first time. DLLME-SFO is automated by hyphenating a sequential injection analysis (SIA) system with a custom-made robotic phase separator. Automated in-syringe DLLME is followed by phase separation in a 3D printed device integrating a Peltier cell set, mounted on a multi axis robotic arm. The combined action of the flow system and the robotic arm is controlled by a single software package, enabling the solidification/melting and collection of the organic phase for further analyte quantification. As proof-of-concept, automated DLLME-SFO was applied to the extraction of parabens followed by separation using liquid chromatography, obtaining LODs between 0.3 and 1.3 mu g L-1 (4 mL of sample extracted in 1 mL of 1-dodecanol: MeOH, 15:85, v-v). The method showed a high reproducibility, obtaining intraday RSDs between 4.6% and 5.8% (n = 6), and interday RSDs between 5.6% and 8.6% (n = 6). The developed method was evaluated for the determination of parabens in water, urine, saliva, and personal care products. (AU)

• FAPESP's process: 17/02147-0 - Single drop chromatography and its coupling to mass spectrometry: instrumental strategies, development of materials, automation and analytical applications
• Grantee: Fernando Mauro Lanças
• Support Opportunities: Research Projects - Thematic Grants
• FAPESP's process: 10/19910-9 - Development of green chemistry strategies on chromatography and application to the evaluation of antibiotics degradation in wastewaters by fixed-bed anaerobic bioreactors
• Grantee: Alvaro José dos Santos Neto
• Support Opportunities: Regular Research Grants
• FAPESP's process: 16/21950-5 - Automation strategies for miniaturized sample preparation, case: automated online in syringe stirred SDME
• Grantee: Deyber Arley Vargas Medina
• Support Opportunities: Scholarships abroad - Research Internship - Doctorate