Bifidobacterium spp. reshaping in the gut microbiota by low dose of jucara supplementation and hypothalamic insulin resistance in Wistar rats

Author(s)

Jamar, Giovana ^[1] ; Santamarina, Aline Boveto ^[1] ; Mennitti, Lais Vales ^[1] ; Cesar, Helena de Cassia ^[1] ; Oyama, Lila Missae ^[2] ; de Rosso, Veridiana Vera ^[3] ; Pisani, Luciana Pellegrini ^[3]

Affiliation

^[1] Univ Fed Sao Paulo, Programa Posgrad Interdisciplinar Ciencias Saude, Santos, SP - Brazil

^[2] Univ Fed Sao Paulo, Escola Paulista Med, Dept Fisiol Nutr, Sao Paulo, SP - Brazil

^[3] Univ Fed Sao Paulo, Inst Saude & Soc, Dept Biociencias, Santos, SP - Brazil

• Document type: Journal article
• Source: Journal of Functional Foods; v. 46, p. 212-219, JUL 2018.
• Web of Science Citations: 3

Abstract

High-fat diet (HFD) alters gut microbial diversity and drives a rapid development of hypothalamic inflammation, which impairs hypothalamic insulin sensitivity. The addition of berries in the diet may promote beneficial effects in the host. We investigated the potential of jucara with different doses on the reshaping of the microbiota and improvement in the hypothalamic insulin resistance (HIR) induced by acute HFD treatment. We analyzed pro and anti-inflammatory protein content and expression by ELISA (blood and colon) and Western blotting (hypothalamus); gene expression by RT-PCR (colon and stool). Jucara supplementation restored the fecal Bifidobacteria content, reduced colon inflammation and minimized HIR. It also down-regulated IR alpha and up-regulated pAkt and increased pAkt/Akt ratio. The lower dose was protective against the adverse effects of HFD, by enhancing IRa efficiency in the hypothalamus. To our knowledge, this is the first study to elucidate the role of jucara in the HIR. (AU)

• FAPESP's process: 16/14133-0 - Intracellular mechanisms implicated to inflammatory process in obese subjects before and after supplementation juçara pulp
• Grantee: Luciana Pellegrini Pisani
• Support Opportunities: Regular Research Grants