Intracellular mechanisms implicated to inflammatory process in obese subjects before and after supplementation juçara pulp

• Grant number: 16/14133-0
• Support Opportunities: Regular Research Grants
• Duration: May 01, 2017 - April 30, 2019
• Field of knowledge: Health Sciences - Nutrition - Nutrition Biochemistry
• Principal Investigator: Luciana Pellegrini Pisani
• Grantee: Luciana Pellegrini Pisani
• Host Institution: Instituto de Saúde e Sociedade (ISS). Universidade Federal de São Paulo (UNIFESP). Campus Baixada Santista. Santos , SP, Brazil
• Associated researchers: Lila Missae Oyama ; Veridiana Vera de Rosso

Abstract

Introduction: Obesity is established as a public health problem and requires interventions that allow combating them. Installation of chronic subclinical inflammation and epigenetic alterations are related to pathology of this multifactorial disease. Thus the search for foods able to combat and prevent these diseases became necessary. In this sense, the fruit of juçara palm seems to be a food with potential against obesity due to its composition rich in anthocyanins, polyunsaturated fatty acids and dietary fiber. Methods: 60 obese subjects (BMI 30.0 to 39.9 kg / m2) wil be randomized in two groups, use or not fruit pulp supplementation to 6 weeks. Both genders aged between 31 and 59 years will be evaluated resting energy expenditure by indirect calorimetry (Quark), anthropometry (weight, height, BMI, CP, CC and WHtR), food intake (24HR), profile of fatty acids in serum by gas chromatography, epigenetic markers (global DNA methylation in monocytes; protein and gene expression of proteins involved in epigenetic mechanisms: DNMT1, DNMT3a , DNMT3B and MeCP2, and HDAC enzyme activity in monocytes); The monocyte inflammatory profile of TLR4 / NFºB activation cascade by gene expression of the following proteins associated with inflammation: TLR4, TLR2, IL-6R, IL-10R±, TNFR1, AdipoR2, IL-10, IL-6, TNF, -±, Ob-R; and protein expression of the following proteins: TLR4, TLR2, IL-6R, IL-10R±, TNFR1, AdipoR2, Ob-R, MyD88, TRAF6, and all fractions and phosphorylated IKK ± / ² and NF-kB (p50 subunits and p65). (AU)

Scientific publications

(8)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

JAMAR, GIOVANA; SANTAMARINA, ALINE BOVETO; DIAS, GABRIELA CORREIA; LANDI MASQUIO, DEBORAH CRISTINA; DE ROSSO, VERIDIANA VERA; PISANI, LUCIANA PELLEGRINI. Relationship between fatty acids intake and Clostridium coccoides in obese individuals with metabolic syndrome. Food Research International, v. 113, p. 86-92, NOV 2018. (16/14133-0)

SANTAMARINA, ALINE BOVETO; JAMAR, GIOVANA; MENNITTI, LAIS VALES; CESAR, HELENA DE CASSIA; DE ROSSO, VERDIANA VERA; VASCONCELOS, JOSE RONNIE; OYAMA, LILA MISSAE; PISANI, LUCIANA PELLEGRINI. Supplementation of Jucara Berry (Euterpe edulis Mart.) Modulates Epigenetic Markers in Monocytes from Obese Adults: A Double-Blind Randomized Trial. NUTRIENTS, v. 10, n. 12, DEC 2018. (15/13875-0, 16/14133-0)

JAMAR, GIOVANA; SANTAMARINA, ALINE BOVETO; CASAGRANDE, BRENO PICIN; ESTADELLA, DEBORA; DE ROSSO, VERIDIANA VERA; WAGNER, ROGER; FAGUNDES, MARIANE BITTENCOURT; PISANI, LUCIANA PELLEGRINI. Prebiotic potencial of jucara berry on changes in gut bacteria and acetate of individuals with obesity. EUROPEAN JOURNAL OF NUTRITION, v. 59, n. 8, FEB 2020. (16/14133-0)

SANTAMARINA, ALINE BOVETO; JAMAR, GIOVANA; MENNITTI, LAIS VALES; RIBEIRO, DANIEL ARAKI; CARDOSO, CAROLINE MARGONATO; DE ROSSO, VERIDIANA VERA; OYAMA, LILA MISSAE; PISANI, LUCIANA PELLEGRINI. Polyphenols-Rich Fruit (Euterpe edulis Mart.) Prevents Peripheral Inflammatory Pathway Activation by the Short-Term High-Fat Diet. Molecules, v. 24, n. 9, MAY 1 2019. (18/23623-7, 15/13875-0, 16/14133-0)

DE SOUZA MESQUITA, LEONARDO M.; MENNITTI, V, LAIS; DE ROSSO, VERIDIANA V.; PISANI, LUCIANA P.. The role of vitamin A and its pro-vitamin carotenoids in fetal and neonatal programming: gaps in knowledge and metabolic pathways. NUTRITION REVIEWS, v. 79, n. 1, p. 76-87, JAN 2021. (19/09359-8, 16/18910-1, 16/23242-8, 16/14133-0)

PISANI, LUCIANA PELLEGRINI. The Use of Jucara (Euterpe edulis Mart.) Supplementation for Suppression of NF-B Pathway in the Hypothalamus after High-Fat Diet in Wistar Rats. Molecules, v. 23, n. 7, JUL 2018. (16/14133-0, 15/13875-0)

JAMAR, GIOVANA; SANTAMARINA, ALINE BOVETO; MENNITTI, LAIS VALES; CESAR, HELENA DE CASSIA; OYAMA, LILA MISSAE; DE ROSSO, VERIDIANA VERA; PISANI, LUCIANA PELLEGRINI. Bifidobacterium spp. reshaping in the gut microbiota by low dose of jucara supplementation and hypothalamic insulin resistance in Wistar rats. Journal of Functional Foods, v. 46, p. 212-219, JUL 2018. (16/14133-0)

JAMAR, GIOVANA; SANTAMARINA, ALINE BOVETO; FLYGARE, ANA CAROLINA; GAGLIARDI, ANTONIO; DE ROSSO, VERIDIANA VERA; DOURADO, VICTOR ZUNIGA; PISANI, LUCIANA PELLEGRINI. Effects of the jucara fruit supplementation on metabolic parameters in individuals with obesity: a double-blind randomized controlled trial. JOURNAL OF NUTRITIONAL BIOCHEMISTRY, v. 83, SEP 2020. (16/14133-0)