| Full text | |
| Author(s): |
Santos, G.
[1]
;
Bottino, M. P.
[1]
;
Santos, A. P. C.
[1]
;
Simoes, L. M. S.
[1]
;
Souza, J. C.
[2]
;
Ferreira, M. B. D.
[3]
;
da Silveira, J. C.
[4]
;
Avila, A. C. F. C. M.
[4]
;
Bride, A.
[4]
;
Sales, J. N. S.
[1]
Total Authors: 10
|
| Affiliation: | [1] Univ Fed Lavras, Dept Vet Med, Lavras, MG - Brazil
[2] Univ Fed Lavras, Dept Anim Sci, Lavras, MG - Brazil
[3] Empresa Pesquisa Agr Minas Gerais EPAMIG, Belo Horizonte, MG - Brazil
[4] Univ Sao Paulo, Dept Mol Biol, Sao Paulo - Brazil
Total Affiliations: 4
|
| Document type: | Journal article |
| Source: | Theriogenology; v. 119, p. 214-219, OCT 1 2018. |
| Web of Science Citations: | 0 |
| Abstract | |
The objective was to evaluate the effect of mastitis by somatic cell count (SCC) on follicular growth, ovulation, oocytes and cumulus cells quality and on the concentration and size of exosomes in follicular fluid of dairy cows. In the study, crossbred cows (Bos taurus - Holstein x Bos indicus - Gir) were classified for analysis as Control (SCC<200.000 cells/mL) and Mastitis (SCC>400.000 cells/mL) groups. In experiment 1 (follicular dynamics), cows (n = 57) were submitted to ultrasound evaluations every 24 h, from progesterone-releasing-intravaginal-device (PRID) removal (D8) until 48 h later (D10). Thereafter, evaluations were performed every 12 h, until ovulation or up to 96 h after PRID removal. In experiment 2 (oocyte, cumulus complexes, and follicular fluid evaluation), cows (n = 26) were submitted to follicular aspiration (OPU) for oocyte quality and cumulus cells transcript evaluation. The amount of cumulus complexes transcripts (BCL2, BAX, PI3K, MEN, FOXO3) was determined by Real-Time Polymerase Chain Reaction. Moreover, seven days after the OPU session, the dominant follicle was aspirated. Exosomes were isolated from the follicular fluid for evaluation of particle size and concentration. Ovulation rate {[}Control 77.4% (24/31) and Mastitis 57.7% (15/26); P= 0.09] and viable oocytes rate {[}Control 59.1% (130/220) and Mastitis 41.9% (125/298); P = 0.01] were higher in Control animals. Additionally, there was a greater number of degenerate oocytes {[}Control 6.7 +/- 1.2 and Mastitis 13.3 +/- 5.5; (P = 0.001)] in sub-clinical mastitis cows. There was greater abundance (P = 0.003) of BAX cumulus cell transcripts and exosome mean (P = 0.03) and mode (P = 0.02) was smaller in subclinical mastitis cows. In conclusion, ovulation rate, oocyte quality, and exosome diameter were smaller in cows with SCC>400.000 cells/mL (C) 2018 Published by Elsevier Inc. (AU) | |
| FAPESP's process: | 14/22887-0 - Cell-secreted vesicles containing miRNAs modulate epigenetic changes during in vitro culture of bovine gametes and embryos |
| Grantee: | Juliano Coelho da Silveira |
| Support Opportunities: | Research Grants - Young Investigators Grants |
| FAPESP's process: | 15/21829-9 - Multi-user equipment approved in grant 2014/22887-0: Nanosight LM10 |
| Grantee: | Juliano Coelho da Silveira |
| Support Opportunities: | Multi-user Equipment Program |