Characterization of Skeletal Muscle Strain Lesion Induced by Stretching in Rats: Effects of Laser Photobiomodulation

Author(s)

Ramos, Luciano ^[1] ; Marcos, Rodrigo Labat ^[2] ; Torres-Silva, Romildo ^[3] ; Pallota, Rodney Capp ^[2] ; Magacho, Tatiana ^[1] ; Pazello Mafra, Fernando Francisco ^[3] ; Macedo, Michel Monteiro ^[3] ; de Paiva Carvalho, Rodrigo Leal ^[4] ; Bjordal, Jan Magnus ^[5] ; Lopes-Martins, Rodrigo Alvaro B. ^[6]

Affiliation

^[1] Governador Jones dos Santos Neves, Fac Pitagoras Guarapari Rod, Guarapari, ES - Brazil

^[2] Univ Nove de Julho, Postgrad Program Biophoton Appl Hlth Sci, Sao Paulo - Brazil

^[3] Univ Mogi das Cruzes, Postgrad Program Biomed Engn, Technol Res Ctr NPT, Mogi das Cruzes - Brazil

^[4] Univ Sagrado Coracao, Lab Pesquisa Fisioterapia, PPG Fisioterapia, Sao Paulo - Brazil

^[5] Univ Bergen, Dept Hlth Sci, Bergen - Norway

^[6] Univ Vale do Paraiba UNIVAP, Inst Pesquisa & Desenvolvimento, Sao Paulo - Brazil

• Document type: Journal article
• Source: Photomedicine and Laser Surgery; v. 36, n. 9, p. 460-467, SEP 2018.
• Web of Science Citations: 1

Abstract

Background: Unusual and exhaustive physical exercise can lead to muscle lesions depending on the type of contraction, intensity, duration, age, and level of conditioning. Different therapies have been proposed to prevent or reduce exercise-induced muscle damage. Objective: In this study, we investigate the effects of low-level laser therapy on skeletal muscle strain in an experimental model in rats. Materials and methods: Male Wistar rats (200g) were used. The animals were randomized into groups of six animals. We performed tibialis muscle elongation using a previously described protocol. The animals were anesthetized and submitted to passive stretching of the anterior tibial muscle attached to a weight corresponding to 150% of the body mass of the animal for 20min, rested for 3min, and received a second traction for 20min. The cytokines, tumor necrosis factor- (TNF-), interleukin-1 (IL-1), IL-6, and IL-10, edema, and C-reactive protein (CRP) levels were determined in the tibialis anterior muscle. Results: Plasma extravasation of groups treated with different doses of laser energy, lesion +1J (2.61 +/- 0.46), lesion +3J (2.33 +/- 0.13), lesion +6J (2.92 +/- 0.91), and lesion +9J (2.80 +/- 0.55), shows a significant reduction of extravasation when compared with the injury group (5.46 +/- 1.09). Laser therapy was able to significantly reduce CRP and cytokine levels (TNF-, IL-1, IL-6, and IL-10). Conclusions: Laser photobiomodulation reduced skeletal muscle edema as well as cytokines and CRP, leading to a significant reduction in inflammatory markers. (AU)

• FAPESP's process: 11/18330-1 - The effect of low level laser therapy, topical antiinflammatory drug and exercise in the treatment of papain-induced knee osteoarthritis in rats
• Grantee: Rodrigo Alvaro Brandão Lopes Martins
• Support Opportunities: Regular Research Grants
• FAPESP's process: 13/07276-1 - CEPOF - Optics and Photonic Research Center
• Grantee: Vanderlei Salvador Bagnato
• Support Opportunities: Research Grants - Research, Innovation and Dissemination Centers - RIDC