Photobiomodulation of a flowable matrix in a human skin ex vivo model demonstrates energy-based enhancement of engraftment integration and remodeling

Author(s)

Neves, Lia M. G. ^[1] ; Parizotto, Nivaldo A. ^{[2, 3, 4]} ; Cominetti, Marcia R. ^[1] ; Bayat, Ardeshir ^[5]

Affiliation

^[1] Univ Fed Sao Carlos, Dept Gerontol, Lab Biol Aging LABEN, BR-13565905 Sao Carlos, SP - Brazil

^[2] Univ Fed Sao Carlos, Phys Therapy Dept, Sao Carlos, SP - Brazil

^[3] Univ Araraquara, Biotechnol Postgrad Program, Araraquara - Brazil

^[4] Univ Brazil, Postgrad Biomed Engn, Sao Paulo, SP - Brazil

^[5] Univ Manchester, Div Musculoskeletal & Dermatol Sci, Sch Biol Sci & Hlth, Manchester, Lancs - England

• Document type: Journal article
• Source: Journal of Biophotonics; v. 11, n. 9 SEP 2018.
• Web of Science Citations: 0

Abstract

The use of dermal substitutes to treat skin defects such as ulcers has shown promising results, suggesting a potential role for skin substitutes for treating acute and chronic wounds. One of the main drawbacks with the use of dermal substitutes is the length of time from engraftment to graft take, plus the risk of contamination and failure due to this prolonged integration. Therefore, the use of adjuvant energy-based therapeutic modalities to augment and accelerate the rate of biointegration by dermal substitute engraftments is a desirable outcome. The photobiomodulation (PBM) therapy modulates the repair process, by stimulating cellular proliferation and angiogenesis. Here, we evaluated the effect of PBM on a collagen-glycosaminoglycan flowable wound matrix (FWM) in an ex vivo human skin wound model. PBM resulted in accelerated rate of re-epithelialization and organization of matrix as seen by structural arrangement of collagen fibers, and a subsequent increased expression of alpha-smooth muscle actin (alpha-SMA) and vascular endothelial growth factor A (VEGF-A) leading to an overall improved healing process. The use of PBM promoted a beneficial effect on the rate of integration and healing of FWM. We therefore propose that the adjuvant use of PBM may have utility in enhancing engraftment and tissue repair and be of value in clinical practice. (AU)

• FAPESP's process: 16/24907-3 - Effects of photobiomodulation and Integra® dermal template supplemented with polyssacharide fraction of Lycium barbarum in an ex vivo wound healing model
• Grantee: Lia Mara Grosso Neves
• Support Opportunities: Scholarships abroad - Research Internship - Doctorate