Stress-related salivary proteins affect the production of volatile sulfur compounds by oral bacteria

Author(s)

de Lima, Patricia Oliveira ^[1] ; Nani, Bruno Dias ^[1] ; Almeida, Barbara ^[1] ; Marcondes, Fernanda Klein ^[1] ; Groppo, Francisco Carlos ^[1] ; Alves de Moraes, Antonio Bento ^[1] ; Franz-Montan, Michelle ^[1] ; Cogo-Mueller, Karina ^{[2, 1]}

Affiliation

^[1] Univ Campinas UNICAMP, Dept Physiol Sci, Piracicaba Dent Sch, Piracicaba, SP - Brazil

^[2] Univ Campinas Unicamp, Fac Pharmaceut Sci, Campinas, SP - Brazil

• Document type: Journal article
• Source: ORAL DISEASES; v. 24, n. 7, p. 1358-1366, OCT 2018.
• Web of Science Citations: 1

Abstract

ObjectiveTo determine whether stress-related substances and sex hormones influence the growth and in vitro production of volatile sulfur compounds (VSCs) by Solobacterium moorei and Fusobacterium nucleatum. Materials and MethodsBacteria growth and VSCs production were evaluated in the presence of alpha-amylase, beta-defensin-2, mucin, estradiol, and progesterone. Growth was evaluated by colony counting, and the production of the VSCs hydrogen sulfide (H2S) and methyl mercaptan (CH3SH) was measured using the Oral Chroma instrument. ResultsMucin induced the production of H2S by both bacteria, but had a slight inhibitory effect on CH3SH production by F.nucleatum. It also increased the viability of F.nucleatum. Alpha-amylase increased H2S production by S.moorei and CH3SH production by F.nucleatum, but had no effect on H2S production by F.nucleatum. No substance altered the viability of S.moorei. No effects of beta-defensin-2, estradiol, or progesterone were observed. ConclusionThe salivary stress-related proteins mucin and alpha-amylase altered VSCs production by F.nucleatum and S.moorei, favoring H2S production. These findings are a step toward understanding the relation between stress and increased amounts of H2S. (AU)

• FAPESP's process: 13/26691-0 - Relationship between stress and oral microbiota: the effect on the production of volatile sulphur compounds and the role of beta-defensin
• Grantee: Bruno Dias Nani
• Support Opportunities: Scholarships in Brazil - Master
• FAPESP's process: 11/50419-2 - Oral health and behavior: interdisciplinary perspectives and emotional aspects in the health-disease process
• Grantee: Antonio Bento Alves de Moraes
• Support Opportunities: Research Projects - Thematic Grants
• FAPESP's process: 14/11481-2 - Relationship between stress, female sex hormones and oral microbiota: effect on volatile sulfur compounds production and beta-defensin role
• Grantee: Patricia Oliveira de Lima
• Support Opportunities: Scholarships in Brazil - Post-Doctoral