| Full text | |
| Author(s): |
Midoux, Cedric
[3]
Total Authors: 1
|
| Affiliation: | [1] Irstea, UR HBAN, F-92761 Antony - France
Total Affiliations: 1
|
| Document type: | Journal article |
| Source: | Science of The Total Environment; v. 649, p. 482-494, FEB 1 2019. |
| Web of Science Citations: | 1 |
| Abstract | |
Three distinct biological reactors fed with synthetic medium (UASB_Control), synthetic medium and linear alkylbenzene sulfonate (LAS; UASB_SL), and real laundry wastewater (UASB_LW) were compared using a metatranscriptomic approach to determine putative bioindicator genes and taxonomies associated to all steps of anaerobic LAS biodegradation pathway. A homemade bioinformatics pipeline combined with an R workflow was developed to perform the RNAseq data analysis. UASB_SL and UASB_LW showed similar values of LAS biological degradation (-47%) and removal (53-55%). Rarefaction analysis revealed that 1-2 million reads were sufficient to access the whole functional capacity. In the first step of LAS biodegradation pathway, fumarate reductase subunit C was detected and taxonomically assigned to the genus Syntrophobacter (0.002% - UASB_SL; 0.0015% - UASB_LW; not detected - UASB_Control). In the second step, many enzymes related to beta-oxidation were observed and most of them with low relative abundance in UASB Control and taxonomically related with Smithella. Acinetobacter and Syntrophorhabdus. For the ring cleavage step, the abundance of 6 OCI-I CoA hydrolase putative gene was ten times higher in UASB_SL and UASB_LW when compared to UASB_Control, and assigned to Desulfomonile and Syntrophorhabdus. Finally, the adenylylsulfate reductase, taxonomically related with Desulfovibrio and Desulfomonile, was observed in the desulfonation step with the highest relative abundance in UASB_LW. (C) 2018 Elsevier B.V. All rights reserved. (AU) | |
| FAPESP's process: | 14/16426-0 - Metagenomic of microbiota of anaerobic reactors applied in anionic surfactant degradation in commercial laundry wastewater |
| Grantee: | Tiago Palladino Delforno |
| Support Opportunities: | Scholarships in Brazil - Post-Doctoral |
| FAPESP's process: | 17/00817-8 - Mining meta omic data from anaerobic digestion bioprocesses |
| Grantee: | Tiago Palladino Delforno |
| Support Opportunities: | Scholarships abroad - Research Internship - Post-doctor |