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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Comparative metatranscriptomic analysis of anaerobic digesters treating anionic surfactant contaminated wastewater

Full text
Author(s):
Midoux, Cedric [3]
Total Authors: 1
Affiliation:
[1] Irstea, UR HBAN, F-92761 Antony - France
Total Affiliations: 1
Document type: Journal article
Source: Science of The Total Environment; v. 649, p. 482-494, FEB 1 2019.
Web of Science Citations: 1
Abstract

Three distinct biological reactors fed with synthetic medium (UASB_Control), synthetic medium and linear alkylbenzene sulfonate (LAS; UASB_SL), and real laundry wastewater (UASB_LW) were compared using a metatranscriptomic approach to determine putative bioindicator genes and taxonomies associated to all steps of anaerobic LAS biodegradation pathway. A homemade bioinformatics pipeline combined with an R workflow was developed to perform the RNAseq data analysis. UASB_SL and UASB_LW showed similar values of LAS biological degradation (-47%) and removal (53-55%). Rarefaction analysis revealed that 1-2 million reads were sufficient to access the whole functional capacity. In the first step of LAS biodegradation pathway, fumarate reductase subunit C was detected and taxonomically assigned to the genus Syntrophobacter (0.002% - UASB_SL; 0.0015% - UASB_LW; not detected - UASB_Control). In the second step, many enzymes related to beta-oxidation were observed and most of them with low relative abundance in UASB Control and taxonomically related with Smithella. Acinetobacter and Syntrophorhabdus. For the ring cleavage step, the abundance of 6 OCI-I CoA hydrolase putative gene was ten times higher in UASB_SL and UASB_LW when compared to UASB_Control, and assigned to Desulfomonile and Syntrophorhabdus. Finally, the adenylylsulfate reductase, taxonomically related with Desulfovibrio and Desulfomonile, was observed in the desulfonation step with the highest relative abundance in UASB_LW. (C) 2018 Elsevier B.V. All rights reserved. (AU)

FAPESP's process: 14/16426-0 - Metagenomics of microbiota of anaerobic reactors applied in anionic surfactant degradation in commercial laundry wastewater
Grantee:Tiago Palladino Delforno
Support Opportunities: Scholarships in Brazil - Post-Doctoral
FAPESP's process: 17/00817-8 - Mining meta omic data from anaerobic digestion bioprocesses
Grantee:Tiago Palladino Delforno
Support Opportunities: Scholarships abroad - Research Internship - Post-doctor