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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Hydroperoxide and carboxyl groups preferential location in oxidized biomembranes experimentally determined by small angle X-ray scattering: Implications in membrane structure

Full text
Author(s):
De Rosa, Raffaela [1] ; Spinozzi, Francesco [2] ; Itri, Rosangela [1]
Total Authors: 3
Affiliation:
[1] Univ Sao Paulo, Inst Phys, Sao Paulo, SP - Brazil
[2] Polytech Univ Marche, Dept Life & Environm Sci, Ancona - Italy
Total Affiliations: 2
Document type: Journal article
Source: BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES; v. 1860, n. 11, p. 2299-2307, NOV 2018.
Web of Science Citations: 3
Abstract

We report small angle X-ray scattering (SAXS) data from large unilamellar vesicles as model membranes composed of 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocoline (POPC) and two oxidized species, namely its hydroperoxidized form POPC-OOH and 1-palmitoyl-2-azelaoyl-sn-glycero-3-phosphocholine (PazePC) lipid that has a carboxyl group at the end of its truncated sn-2 chain. The replacement of POPC by either POPC-OOH (POPC-OOHxPOPC1-x or PazePC (PazePC(x)POPC(1-x)), with oxidized lipid molar ratio x varying from 0.00 up to 1.00, permits to experimentally inspect changes in the membrane structural properties due to oxidation. The volume fraction distribution of each lipid chemical group along the bilayer is determined. The results quantify that 95% of the hydroperoxide group lies in the membrane polar moiety, near the carbonyl and phosphate groups, whereas just 5% of OOH group experiences the polar/apolar interface, for all values of x studied. In the case of PazePC up to x = 0.33, a bimodal distribution of the carboxyl group in the interior and polar regions of the lipid membrane is obtained, probably due to a dynamic movement of the shortened alkyl chain towards the water interface. The mean molecular area A gradually increases from 65.4 +/- 0.4 angstrom(2) for POPC bilayers to 78 +/- 2 angstrom(2) for pure POPC-OOH bilayers, whereas POPC-OOH membrane thickness resulted to be 20% thinner than the non oxidized POPC membrane. For PazePC up to x = 0.33, A increases to 67 +/- 2 angstrom(2) with 10% of membrane thinning. The SAXS results thus demonstrate how the lipid oxidation progress affects the membrane structural features, thus paving the way to better understand membrane damage under oxidative stress. (AU)

FAPESP's process: 12/50680-5 - Photosensitization in life sciences
Grantee:Mauricio da Silva Baptista
Support type: Research Projects - Thematic Grants
FAPESP's process: 14/02511-5 - Physical damage on model membranes promoted by photosensitization
Grantee:Raffaela de Rosa
Support type: Scholarships in Brazil - Doctorate (Direct)
FAPESP's process: 16/25847-4 - Structural studies of water dispersion of lipids by X-ray scattering techniques: advances in data analysis by the Genfit software
Grantee:Rosangela Itri
Support type: Research Grants - Visiting Researcher Grant - International