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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

An antibody-based platform for melatonin quantification

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Author(s):
Brazaca, Lais C. [1] ; Bramorski, Camila B. [1] ; Cancino-Bernardi, Juliana [1] ; Cruz-Machado, Sanseray da Silveira [2] ; Markus, Regina P. [2] ; Janegitz, Bruno C. [3] ; Zucolotto, Valtencir [1]
Total Authors: 7
Affiliation:
[1] Univ Sao Paulo, Sao Carlos Inst Phys, Nanomed & Nanotoxicol Grp, BR-13560970 Sao Carlos, SP - Brazil
[2] Univ Sao Paulo, Inst Biosci, Dept Physiol, Lab Chronopharmacol, BR-05508090 Sao Paulo, SP - Brazil
[3] Univ Fed Sao Carlos, Dept Nat Sci Math & Educ, BR-13600970 Araras, SP - Brazil
Total Affiliations: 3
Document type: Journal article
Source: COLLOIDS AND SURFACES B-BIOINTERFACES; v. 171, p. 94-100, NOV 1 2018.
Web of Science Citations: 1
Abstract

Melatonin, the `chemical signal of darkness', is responsible to regulate biological rhythms and different physiological processes. It is mainly produced by the pineal gland as a hormone in a rhythmic daily basis, but it may also be synthesized by other tissues, such as immune cells, under inflammatory conditions. Its abnormal circulating levels have been related to several diseases such as type 2 diabetes, Alzheimer's disease and some types of cancer. Currently, melatonin is exclusively quantified by ELISA or radioimmunoassays, which although are very sensitive techniques and present low detection limits, usually require specialized personal and equipment, restricting the tests to a limited number of patients. To overcome such limitations, we developed a novel easy-to use electrochemical immunosensor for rapid melatonin quantification. Anti-melatonin antibodies were immobilized into Indium tin oxide (ITO) platforms using (3-Aminopropyl)triethoxysilane (APTES), 1-ethyl-3-(3-dimethylaminopropyl)carboctiimide (EDC) and N-hydroxysuccinimide (NHS) crosslinkers. The platforms were assayed with synthetic and biologically-present melatonin containing samples. The developed device displayed a linear response in the concentration range from 0.75 to 7.5 mu mol/L and a limit of detection of 0.175 mu mol/L using Electrochemical Impedance Spectroscopy (EIS) (R-2 = 0.989) and 0313 mu mol/L using Cyclic Voltammetry (CV) (R-2 = 0.953) for synthetic melatonin. Furthermore, the sensors exhibited a good stability and reproducibility (3,45% and 2.87% for EIS and CV, respectively, n = 3), maintaining adequate response even after 30 days of assembly. On biologically-present melatonin-containing samples the device displayed a similar performance when compared to ELISA technique (deviation of 13.31%). We expect that the developed device contributes significantly to the medical area allowing precise and complete diagnosis of the diseases related to abnormal levels of melatonin. (AU)

FAPESP's process: 13/13691-1 - Immune-pineal axis: time-niology integrated to surveillance and defense
Grantee:Regina Pekelmann Markus
Support Opportunities: Research Projects - Thematic Grants
FAPESP's process: 15/02623-0 - New Platforms for Early and Rapid Alzheimer's disease diagnosis
Grantee:Laís Canniatti Brazaca
Support Opportunities: Scholarships in Brazil - Doctorate
FAPESP's process: 15/19099-2 - Development of electrochemical sensors and biosensors for different analytical purposes
Grantee:Bruno Campos Janegitz
Support Opportunities: Regular Research Grants