Distinct features of rabbit and human adipose-derived mesenchymal stem cells: implications for biotechnology and translational research

Author(s)

Zomer, Helena Debiazi ^{[1, 2]} ; Roballo, Kelly C. S. ^[1] ; Lessa, Thais Borges ^[1] ; Bressan, Fabiana Fernandes ^[1] ; Goncalves, Natalia Nardeli ^[1] ; Meirelles, Flavio Vieira ^{[1, 3]} ; Trentin, Andrea Goncalves ^[2] ; Ambrosio, Carlos Eduardo ^{[1, 3]}

Affiliation

^[1] Univ Sao Paulo, Fac Vet Med & Anim Sci, Dept Surg, Sect Anat, Sao Paulo - Brazil

^[2] Santa Catarina Fed Univ UFSC, Fac Biol Sci, Dept Cell Biol Embryol & Genet, Florianopolis, SC - Brazil

^[3] Univ Sao Paulo, Fac Anim Sci & Food Engn, Dept Vet Med, Ave Duque de Caxias Norte 225, BR-13635900 Sao Paulo - Brazil

• Document type: Journal article
• Source: STEM CELLS AND CLONING-ADVANCES AND APPLICATIONS; v. 11, p. 43-54, 2018.
• Web of Science Citations: 3

Abstract

Introduction: Owing to their similarity with humans, rabbits are useful for multiple applications in biotechnology and translational research from basic to preclinical studies. In this sense, mesenchymal stem cells (MSCs) are known for their therapeutic potential and promising future in regenerative medicine. As many studies have been using rabbit adipose-derived MSCs (ASCs) as a model of human ASCs (hASCs), it is fundamental to compare their characteristics and understand how distinct features could affect the translation to human medicine. Objective: The aim of this study was to comparatively characterize rabbit ASCs (rASCs) and hASCs to further uses in biotechnology and translational studies. Materials and methods: rASCs and hASCs were isolated and characterized by their immunophenotype, differentiation potential, proliferative profile, and nuclear stability in vitro. Results and discussion: Both ASCs presented differentiation potential to osteocytes, chondrocytes, and adipocytes and shared similar immunophenotype expression to CD105+, CD34-, and CD45-, but rabbit cells expressed significantly lower CD73 and CD90 than human cells. In addition, rASCs presented greater clonogenic potential and proliferation rate than hASCs but no difference in nuclear alterations. Conclusion: The distinct features of rASCs and hASCs can positively or negatively affect their use for different applications in biotechnology (such as cell reprogramming) and translational studies (such as cell transplantation, tissue engineering, and pharmacokinetics). Nevertheless, the particularities between rabbit and human MSCs should not prevent rabbit use in preclinical models, but care should be taken to interpret results and properly translate animal findings to medicine. (AU)

• FAPESP's process: 13/09392-9 - Production of induced pluripotent stem cells as model studies in rabbits
• Grantee: Carlos Eduardo Ambrósio
• Support Opportunities: Regular Research Grants
• FAPESP's process: 17/21266-0 - Amniotic Progenitor cells and therapeutics potention
• Grantee: Carlos Eduardo Ambrósio
• Support Opportunities: Regular Research Grants