| Full text | |
| Author(s): |
Eiras, Matheus Credendio
[1]
;
Miranda-Furtado, Cristiana Libardi
[1, 2]
;
Fernandes, Artur
[1]
;
Ramos, Ester Silveira
[1]
Total Authors: 4
|
| Affiliation: | [1] Univ Sao Paulo, Ribeirao Preto Med Sch, Dept Genet, Sao Paulo, SP - Brazil
[2] Univ Fed Ceara, Drug Res & Dev Ctr, Fortaleza, Ceara - Brazil
Total Affiliations: 2
|
| Document type: | Journal article |
| Source: | Theriogenology; v. 122, p. 84-87, DEC 2018. |
| Web of Science Citations: | 0 |
| Abstract | |
Birds have a ZZ and ZW sex chromosome system (male and female, respectively). On the short arm of the Z chromosome of Gallus gallus domesticus there is a repetitive region called MHM region, which is absent on the W chromosome, that causes a natural copy number difference of the MHM region between the sexes, making possible the development of a quantitative PCR (qPCR) sexing assay, based on the Z chromosome double dose. Twenty-seven samples of tissues from eight adult Gallus gallus domesticus (four males and four females) were used to establish the parameters of the MHM copy-number sexing assay. We blinded sexed 20 chicks using 140 samples from different tissues (heart, brain, gonad, kidney, lung, muscle, and blood). The success rate of the assay was 100% (140/140). It required small amounts of DNA (0.39 ng), opening the perspective to the use of the assay in studies in which the amount of available DNA is limited. (C) 2018 Published by Elsevier Inc. (AU) | |
| FAPESP's process: | 09/08313-2 - Methylation pattern and differential MHM region expression during chicken gonadal development |
| Grantee: | Ester Silveira Ramos |
| Support Opportunities: | Regular Research Grants |