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Biotechnological advances in sperm processing


Sperm sexing to obtain semen doses with a high percentage of gamete carriers X or Y chromosome, even more relevant the role of artificial insemination in maximizing genetic progress between generations; productivity and economic return in some farming activities; assisted reproduction in mammals; and genetic counseling of the human species. The objectives of this project will be study the sperm content X or Y chromosomes of cattle to select sex-specific proteins; identifying amino acid residues of interest proteins by mass spectrometry; produce sex-specific antibodies by heterologous immunization (chickens); and verifying the binding efficiency of these antibodies to bovine sperm. Differentials proteins encountered by mass spectrometry will be purified by liquid chromatography and tested for binding efficiency at 50% of cells of a non-sexed semen using flow cytometry, which checks the connection of the anti-protein antibody of interest. (AU)

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Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
CARNEIRO, JOAO A. M.; CANISSO, IGOR F.; BANDEIRA, R. S.; SCHEEREN, V. F. C.; FREITAS-DELL'AQUA, CAMILA P.; ALVARENGA, MARCO A.; PAPA, FREDERICO O.; DELL'AQUA, JR., JOSE A.. Effects of coenzyme Q10 on semen cryopreservation of stallions classified as having good or bad semen freezing ability. Animal Reproduction Science, v. 192, p. 107-118, . (15/25638-3)
SCOTT, CAROLINE; DE SOUZA, FABIANA F.; ARISTIZABAL, VIVIANA H. V.; HETHRINGTON, LOUISE; KRISP, CHRISTOPH; MOLLOY, MARK; BAKER, MARK A.; DELL'AQUA JUNIOR, JOSE ANTONIO. Proteomic profile of sex-sorted bull sperm evaluated by SWATH-MS analysis. Animal Reproduction Science, v. 198, p. 121-128, . (13/23351-3, 15/25638-3, 15/25698-6)
NOVELLO, GUILHERME; SEGABINAZZI, LORENZO G. T. M.; LISBOA, FERNANDO P.; CANUTO, LUCAS E.; FREITAS-DELL'AQUA, CAMILLA P.; DELL'AQUA JR, JOSE A.; CANISSO, IGOR F.. High or Low Body Fat Deposition in the Presence of a Normal Oral Sugar Test is Not Associated With Postthaw Semen Parameters in Stallions. Journal of Equine Veterinary Science, v. 95, . (15/25638-3)

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