EMU científico: acquisition of a mass spectrometry platform for the advanced characterization of protein glycosylation and structure in healthy and disease conditions

• Grant number: 22/11334-6
• Support Opportunities: Research Infrastructure Program - Scientific
• Start date: August 01, 2023
• End date: July 31, 2030
• Field of knowledge: Biological Sciences - Biochemistry - Chemistry of Macromolecules
• Principal Investigator: Giuseppe Palmisano
• Grantee: Giuseppe Palmisano
• Host Institution: Instituto de Ciências Biomédicas (ICB). Universidade de São Paulo (USP). São Paulo , SP, Brazil
• Associated researchers: Fabio Cesar Gozzo ; Gustavo Henrique Goldman ; Suely Kazue Nagahashi Marie
• Associated scholarship(s): 24/20365-8 - Implementation and optimization of computational platforms for expanding the proteome data analysis, BP.TT

Abstract

This proposal aims to implement a multiuser mass spectrometry-based platform specialized in glycobiology and structural proteomics by acquiring an Orbitrap Eclipse Tribrid coupled to a Vanquish Neo liquid chromatographic system. The technical features of this equipment such as different fragmentation techniques, ion mobility separation, MSn acquisition methods, data acquisition versatility, high accuracy, and resolution will improve the identification and quantification of glycoproteins, intact glycopeptides, and crosslinked peptides in complex biological systems. These analyses will aim at understanding the role of glycans and protein conformational changes in pathophysiological processes and their implication as biomarkers and therapeutic targets. The orbitrap Eclipse Tribrid will allow the implementation of analytical strategies for identifying and quantifying these biomolecules and acquiring high-quality data that will benefit postgraduate students, researchers, and companies by increasing international competitiveness. This equipment does not exist in Brazil, and its availability will help advance our glycobiology and structural proteomics knowledge from basic science to biotechnological and translational applications. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

MARTINS, LAYLA FARAGE; DOS SANTOS JUNIOR, ARIOSVALDO PEREIRA; NICASTRO, GIANLUCCA GONCALVES; SCHEUNEMANN, GABY; ANGELI, CLAUDIA BLANES; ROSSI, FERNANDO PACHECO NOBRE; QUAGGIO, RONALDO BENTO; PALMISANO, GIUSEPPE; SGRO, GERMAN GUSTAVO; ISHIDA, KELLY; et al. Phages ZC01 and ZC03 require type-IV pilus for Pseudomonas aeruginosa infection and have a potential for therapeutic applications. MICROBIOLOGY SPECTRUM, v. N/A, p. 17-pg., 2024-10-29. (21/10577-0, 20/04923-0, 18/18257-1, 18/15549-1, 22/11334-6)