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Full text
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Author(s):
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Fernandes, Fabio Henrique
[1, 2]
;
Botasso-Nasciutti, Mario Otavio
[1]
;
Ventura Savio, Andre Luiz
[1]
;
Menezes Souza, Leonardo da Cunha
[1]
;
Fernandes-Cal, Jhennifer Rebecca
[1]
;
Cardoso, Fabio Florenca
[3]
;
de Mattos Fontes, Marcos Roberto
[3]
;
Albuquerque, Anjaina Fernandes
[4]
;
Munari, Carla C.
[1]
;
Kummrow, Fabio
[5]
;
Umbuzeiro, Gisela de Aragao
[4]
;
Favero Salvadori, Daisy Maria
[1, 2]
Total Authors: 12
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| Affiliation: | [1] Sao Paulo State Univ, Med Sch, Dept Pathol, Botucatu, SP - Brazil
[2] Natl Inst Alternat Technol Detect Toxicol Evaluat, Inst Chem Araraquara, Araraquara, SP - Brazil
[3] Sao Paulo State Univ, Biosci Inst, Dept Phys & Biophys, Botucatu, SP - Brazil
[4] Univ Estadual Campinas, Sch Technol, Limeira, SP - Brazil
[5] Univ Fed Sao Paulo, Inst Environm Chem & Pharmaceut Sci, Diadema, SP - Brazil
Total Affiliations: 5
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| Document type: |
Journal article
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| Source: |
Environmental and Molecular Mutagenesis;
v. 59,
n. 9,
p. 822-828,
DEC 2018.
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| Web of Science Citations: |
2
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| Abstract |
Color Index (C.I.) Disperse Red 1 (DR1) is a widely used textile azo dye found in rivers. As it may not be completely removed by conventional treatments, humans can be exposed through drinking water. Studies have supported in vitro toxicity and mutagenicity of commercial DR1. This study aimed to investigate the mutagenic and toxicogenomic effects of commercial DR1 in multiple tissues/organs of Swiss male mice. For that, animals were orally exposed to the dye (by gavage), at single doses of 0.0005, 0.005, 0.5, 50, or 500 mg/kg bw. The two lowest doses were equivalent to the ones found in two Brazilian rivers receiving influx of textile discharges. Cytotoxicity, micronucleated cell frequencies (for all doses tested), primary DNA damage (comet assay), and gene expression profiling of (0.0005 and 0.005 mg/kg of bw) were investigated 24 hr after animal exposure to commercial DR1. Data showed increased frequencies of micronucleated polychromatic erythrocytes in bone marrow cells after treatment with 0.5 and 50 mg/kg bw. At 0.005 mg/kg bw, commercial DR1 induced an increase of primary DNA damage in liver, but not in kidney cells. Additionally, upregulation of genes involved in the inflammatory process (IL1B) (0.0005 and 0.005 mg/kg bw) and cell-cycle control (CDKN1A) in liver cells, and apoptosis (BCL2 and BAX) in leukocytes (0.005 mg/kg bw) were also detected. In conclusion, the commercial DR1 was genotoxic (chromosome aberrations and primary DNA damage) and modulated gene expression in mice, and such effects were dependent on the doses and tissues analyzed. Environ. Mol. Mutagen. 59:822-828, 2018. (c) 2018 Wiley Periodicals, Inc. (AU) |
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| FAPESP's process: |
14/50945-4 - INCT 2014: National Institute for Alternative Technologies of Detection, Toxicological Evaluation and Removal of Micropollutants and Radioactivies
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| Grantee: | Maria Valnice Boldrin |
| Support type: |
Research Projects - Thematic Grants
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| FAPESP's process: |
08/10449-7 - Assessment of occurrence, toxicity/genotoxicity and degradation processes of dyes in effluents and surface water
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| Grantee: | Maria Valnice Boldrin |
| Support type: |
Research Projects - Thematic Grants
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