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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Surface immunoglobulins of erythrocytes and platelets in dogs naturally infected by Rangelia vitalii

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Author(s):
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Franca, Raqueli Teresinha [1] ; Pillat, Micheli Mainardi [2] ; da Silva, Cassia Bagolin [3] ; Schafer, Andressa Salvadori [3] ; Dornelles, Guilherme Lopes [3] ; Costa, Marcio Machado [4] ; Chaves, Rafael Oliveira [1] ; de Andrade, Cinthia Melazzo [3] ; Erhardt, Magnolia Martins [3] ; Antoziazzi, Alfredo Quites [5] ; Ulrich, Henning [2] ; da Silva, Aleksandro Schafer [6] ; dos Anjos Lopes, Sonia Terezinha [3]
Total Authors: 13
Affiliation:
[1] Univ Caxias do Sul, Ctr Life, Rua Francisco Getulio Vargas, 1130, Bloco S, 414, BR-95070560 Caxias Do Sul, RS - Brazil
[2] Univ Sao Paulo, Inst Chem, Dept Biochem, Sao Paulo, SP - Brazil
[3] Univ Fed Santa Maria, Dept Small Anim, Santa Maria, RS - Brazil
[4] Univ Passo Fundo, Dept Vet Med, Passo Fundo, RS - Brazil
[5] Univ Fed Santa Maria, Dept Large Anim, Santa Maria, RS - Brazil
[6] Univ Estado Santa Catarina, Dept Anim Sci, Chapeco, SC - Brazil
Total Affiliations: 6
Document type: Journal article
Source: Microbial Pathogenesis; v. 121, p. 245-251, AUG 2018.
Web of Science Citations: 0
Abstract

Rangelia vitalii is a protozoan of the Babesiidae family that parasitizes domestic and wild dogs in South American countries. The main laboratory findings in blood samples from animals infected by R. vitalii are anemia and thrombocytopenia. The aim of this study was to detect IgM and IgG immunoglobulins on the surface of red blood cells and platelets, as well as to determine the percentage of reticulated platelets and reticulocytes in dogs naturally infected by R. vitalii. Blood samples from twenty dogs seen at the Veterinary Hospital of the Federal University of Santa Maria (UFSM) were divided into two groups: the diseased group consisted of blood samples from 10 animals with the diagnosis of rangeliosis, and the healthy group (control) consisted of samples from 10 healthy animals. All diseased dogs showed normocytic normochromic anemia but showed no differences (p > 0.05) in reticulocyte counts compared to healthy dogs. Moreover, IgM and IgG immunoglobulins were detected on the surface of the plasma membrane of red blood cells from both groups, but the amounts did not differ between groups (p > 0.05). Thrombocytopenia in infected animals was classified as severe. The percentage of reticulated platelets was higher (p < 0.001) in diseased dogs than in healthy animals. Diseased animals showed more IgM immunoglobulins bound to the surface of platelets than did the healthy group (p < 0.001). However, the amount of IgG bound to the surface of platelets was not different between groups. In conclusion, we showed that R. vitalii caused immune-mediated thrombocytopenia since IgM immunoglobulins were found on the surface of platelets of diseased dogs. We suggest that the binding of immunoglobulins on platelet surfaces contributes to early destruction of these cells and, consequently, alterations in hemostasis. (AU)

FAPESP's process: 12/50880-4 - Stem cells: from basic studies of kinin and purinergic receptor roles towards therapeutical applications
Grantee:Alexander Henning Ulrich
Support Opportunities: Research Projects - Thematic Grants
FAPESP's process: 15/19478-3 - Role of kinin-B2 receptor in pathogenesis and progression of familial Alzheimer's Disease: from neurogenesis and immune response to cognition
Grantee:Micheli Mainardi Pillat
Support Opportunities: Scholarships in Brazil - Post-Doctoral