Isolation of Chavibetol and Methyleugenol from Essential Oil of Pimenta pseudocaryophyllus by High Performance Liquid Chromatography

Author(s)

Niculau, Edenilson dos Santos ^{[1, 2]} ; Ribeiro, Leandro do Prado ^[3] ; Ansante, Thiago Felipe ^[4] ; Fernandes, Joao Batista ^[1] ; Forim, Moacir Rossi ^[1] ; Vieira, Paulo Cezar ^[1] ; Vendramim, Jose Djair ^[4] ; das Gracas Fernandes da Silva, Maria Fatima ^[1]

Affiliation

^[1] Univ Fed Sao Carlos DQ UFSCar, Dept Quim, Rd Washington Luis, Km 235, BR-13565905 Sao Carlos, SP - Brazil

^[2] Univ Fed Tocantins, Curso Quim, Ctr Ciencias Integradas, Ave Paraguai S-N, Esquina Com Rua Uxiramas, BR-77824838 Araguaina, TO - Brazil

^[3] Empresa Pesquisa Agr & Extensao Rural Santa Catar, Ctr Pesquisa Agr Familiar, Rua Ferdinando Ricieri Tusset S-N, BR-89801970 Chapeco, SC - Brazil

^[4] Univ Sao Paulo, Escola Super Agr Luiz de Queiroz, Dept Entomol & Acarol, Ave Padua Dias 11, BR-13418900 Piracicaba, SP - Brazil

• Document type: Journal article
• Source: Molecules; v. 23, n. 11 NOV 2018.
• Web of Science Citations: 1

Abstract

A high performance liquid chromatography (HPLC) method was developed for the simultaneous isolation, on a semi-preparative scale, of chavibetol and methyleugenol from the crude essential oil of P. pseudocaryophyllus leaves. The purity of the isolated compounds and their quantifications were developed using GC/FID. Chavibetol was isolated with high purity (98.7%) and mass recovery (94.6%). The mass recovery (86.4%) and purity (85.3%) of methyleugenol were lower than those of chavibetol. Both compounds were identified on the basis of spectral analysis. The results suggest that the method can provide chavibetol with high purity, mass recovery, and productivity from crude essential, which will be used in bioassays against stored insect pests. (AU)

• FAPESP's process: 08/57859-5 - Biorrational control of insect pests
• Grantee: Maria Fátima das Graças Fernandes da Silva
• Support Opportunities: Research Projects - Thematic Grants
• FAPESP's process: 11/22975-8 - Chemical study of Xanthomonas axonopodis pv. citri and its influence on chemical profile of Citrus sinensis
• Grantee: Edenilson dos Santos Niculau
• Support Opportunities: Scholarships in Brazil - Doctorate