Advanced search
Start date
(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Using an Electrochemical Aptasensor to Early Detect Prostate Specific and Free Prostate Specific Antigens Released by Cancer Cells

Full text
Parra, Joao P. R. L. L. [1] ; Crulhas, Bruno P. [1] ; Basso, Caroline R. [1] ; Delella, Flavia K. [1] ; Castro, Gustavo R. [1] ; Pedrosa, Valber A. [1]
Total Authors: 6
[1] UNESP, Inst Biosci, Dept Chem & Biochem, Botucatu, SP - Brazil
Total Affiliations: 1
Document type: Journal article
Source: Electroanalysis; v. 30, n. 12, p. 2869-2877, DEC 2018.
Web of Science Citations: 4

The detection of prostate cancer (PCa) in its early stage is essential to prevent metastasis. Patients who start their treatment earlier have greater chances to be cured; therefore, several research groups have been developing a simple and non-invasive methodology to detect PCa. The aim of this article is to report a new method to detect prostate specific (PSA) and free prostate-specific antigens (PSA) released by cancer cells based on the use of an aptasensor. Scanning electron microscopy (SEM), EDS and cyclic voltammetry were the instruments adopted to assess the surface of the biosensor. According to our results, the aptasensor was highly sensitive and recorded linear response 1-100 ng/ml of PSA and fPSA and detection limit 1.1 ng/ml. Studies on interfering compounds such as human serum protein, ascorbic acid, uric acid and mucin type I have shown low correlation to the used aptamer. Thus, this biosensor could be adopted in the near future to early diagnose PCa. (AU)

FAPESP's process: 16/15919-8 - Monitoring of prostate cancer biomarkers using a biosensor
Grantee:Valber de Albuquerque Pedrosa
Support type: Regular Research Grants
FAPESP's process: 16/25749-2 - Study of prostate cancer biomarkers by aptameric sensors
Grantee:Bruno Pereira Crulhas
Support type: Scholarships in Brazil - Post-Doctorate