Curcumin-mediated antimicrobial photodynamic therapy reduces the viability and vitality of infected dentin caries microcosms

Author(s)

Cusicanqui Mendez, Daniela Alejandra ^[1] ; Gutierres, Eliezer ^[1] ; Dionisio, Evandro Jose ^[1] ; Rabelo Buzalaf, Marilia Afonso ^[2] ; Oliveira, Rodrigo Cardoso ^[2] ; Andrade Moreira Machado, Maria Aparecida ^[1] ; Cruvinel, Thiago ^[1]

Affiliation

^[1] Univ Sao Paulo, Dept Pediat Dent Orthodont & Publ Hlth, Bauru Sch Dent, Alameda Dr Octavio Pinheiro Brisolla 9-75, BR-17012901 Bauru, SP - Brazil

^[2] Univ Sao Paulo, Dept Biol Sci, Bauru Sch Dent, Sao Paulo - Brazil

• Document type: Journal article
• Source: Photodiagnosis and Photodynamic Therapy; v. 24, p. 102-108, DEC 2018.
• Web of Science Citations: 6

Abstract

Background: To our knowledge, there is a lack of evidence on the effect of Antimicrobial Photodynamic Therapy (aPDT) by the application of curcumin against complex biofilms of dental caries lesions. This study aimed to evaluate the viability, vitality, and acid metabolism of infected dentin caries microcosms treated with curcumin-mediated aPDT. Methods: After microcosm biofilms growing anaerobically on bovine dentin disks immersed in McBain medium with 1% sucrose at 37 degrees C for 5 days, the biofilms were treated by the association of DMSO water solution or 600 mu mol L-1 curcumin with 0, 37.5 or 75 J cm(-2) blue LED (455 nm). Then, the colony-forming units (CFU) counts of total microorganisms, total streptococci, mutans streptococci, and total lactobacilli were determined by plating. The lactic acid concentration was analyzed by enzymatic spectrophotometry method, while the vitality of intact biofilms was evaluated by confocal laser scanning microscope (CLSM). Statistical analysis was performed by Kruskal Wallis and post-hoc Dunn's tests (P < 0.05). Results: Curcumin alone did not affect the viability of microorganisms and the vitality of intact biofilms. However, 75 J cm(-2) LED alone decreased the total microorganisms and total lactobacilli counts. The combination of curcumin and LED reduced significantly the counts of all microorganism groups and the vitality of intact biofilms. Differences were not observed between the lactic acid concentrations of distinct groups. Conclusions: Therefore, curcumin-mediated aPDT was effective in reducing the viability and the vitality of infected dentin caries microcosms, without interfering in their acidogenicity. (AU)

• FAPESP's process: 15/08595-9 - Effect of antimicrobial photodynamic therapy on specific microorganisms from dentin caries microcosms
• Grantee: Daniela Alejandra Cusicanqui Méndez
• Support Opportunities: Scholarships in Brazil - Master
• FAPESP's process: 14/10897-0 - Effect of antimicrobial photodynamic therapy on dentin caries microcosms
• Grantee: Thiago Cruvinel da Silva
• Support Opportunities: Regular Research Grants
• FAPESP's process: 14/22238-1 - Effect of antimicrobial photodynamic therapy on dentin caries microcosms
• Grantee: Eliézer Gutierres
• Support Opportunities: Scholarships in Brazil - Scientific Initiation