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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Remote Ischemic Preconditioning Is Efficient in Reducing Hepatic Ischemia-Reperfusion Injury in a Growing Rat Model and Does Not Promote Histologic Lesions in Distant Organs

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Author(s):
Gomes, P. F. M. ; Tannuri, A. C. A. ; Nogueira, T. M. ; Luamoto, L. R. ; Paes, V. R. ; Coelho, M. C. M. ; Goncalves, J. O. ; Serafini, S. ; Tannuri, U.
Total Authors: 9
Document type: Journal article
Source: Transplantation Proceedings; v. 50, n. 10, p. 3840-3844, DEC 2018.
Web of Science Citations: 1
Abstract

Objective. Ischemic preconditioning (IPC) was developed to diminish ischemia-reperfusion injury (IRI). There are two main ways of performing it: direct ischemic-preconditioning (DIP) and remote ischemic-preconditioning (RIP). The objectives of this study were to investigate local and systemic effects of DIP and RIP in liver IRI. Methods. Thirty-two weaning rats (50-70 g body weight; 21 days old) were divided into 4 groups: control (C); ischemia followed by reperfusion (IR); DIP followed by ischemia and reperfusion; and RIP followed by ischemia and reperfusion. In the IR group, the vascular pedicles of medial and left lateral liver lobes were clamped for 60 minutes and then unclamped. In the DIP group, a 10-minute cycle of ischemia followed by a 10-minute reperfusion of the same lobes was performed before 60 minutes of ischemia. In the RIP group, three 5-minute cycles of clamping and unclamping of the femoral vessels were performed before liver ischemia. The animals were euthanized 24 hours after the surgical procedures. Results. The serum levels of liver enzymes were significantly lower in the RIP group compared to the control and IR groups and to the DIP group. The scores of histologic hepatic lesions were significantly lower in RIP animals than those of IR animals (P = .002) and similar to the C group animals. The Bax/BCl-xl relation was lower in the DIP group than that in the RIP group (P = .045) and no differences were observed in histologic analyses of kidney, lung, intestine, and heart. Conclusion. In young animals, the beneficial effects of RIP are more evident than those of DIP. (AU)

FAPESP's process: 16/10689-4 - Evaluation of direct and indirect ischemic pre-conditioning effects in young rat livers
Grantee:Pedro Filipe Medeiros Gomes
Support Opportunities: Scholarships in Brazil - Scientific Initiation