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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

A Tropical Composting Operation Unit at SAo Paulo Zoo as a Source of Bacterial Proteolytic Enzymes

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Author(s):
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Ramos, Patricia L. [1, 2] ; Kondo, Marcia Y. [1] ; Santos, Saara M. B. [1] ; de Vasconcellos, Suzan P. [3] ; Rocha, Rafael C. S. [1, 4] ; da Cruz, Joao B. [2] ; Eugenio, Patricia F. M. [5] ; Cabral, Hamilton [6] ; Juliano, Maria A. [1] ; Juliano, Luiz [1] ; Setubal, Joao C. [7] ; da Silva, Aline M. [7] ; Cappelini, Luciana T. D. [1]
Total Authors: 13
Affiliation:
[1] Univ Fed Sao Paulo, Escola Paulista Med, Dept Biophys, Rua Tres De Maio 100, BR-0404420 Sao Paulo, SP - Brazil
[2] Sao Paulo Zoo Pk Fdn, Appl Res Dept, Ave Miguel Estefano, BR-4241 Sao Paulo, SP - Brazil
[3] Univ Fed Sao Paulo, Dept Biol Sci, Rua Arthur Riedel 275, BR-09972270 Diadema, SP - Brazil
[4] Stoller Brasil, Rod SP 332, Km 138 S-N, BR-13150000 Cosmopolis - Brazil
[5] Univ Sao Paulo, Inst Chem Sao Carlos, Av Trabalhador Sao Carlense 400, BR-13560970 Sao Carlos, SP - Brazil
[6] Univ Sao Paulo, Sch Pharmaceut Sci Ribeirao Preto, Dept Pharmaceut Sci, Av Cafe S-N, BR-14040903 Ribeirao Preto, SP - Brazil
[7] Univ Sao Paulo, Inst Chem, Dept Biochem, Av Prof Lineu Prestes 748, BR-05508000 Sao Paulo, SP - Brazil
Total Affiliations: 7
Document type: Journal article
Source: Applied Biochemistry and Biotechnology; v. 187, n. 1, p. 282-297, JAN 2019.
Web of Science Citations: 2
Abstract

Composting operation systems are valuable sources of microorganisms and enzymes. This work reports the assessment of proteolytic enzymes from cultivable bacteria isolated from a composting facility of the SAo Paulo Zoo Park (SPZPF), SAo Paulo, Brazil. Three hundred bacterial isolates were obtained and identified based on 16S rRNA gene as belonging to 13 different genera. The most common genus among the isolates was Bacillus (67%); some of which show high proteolytic activity in their culture media. Biochemical assays of hydrolytic activities using FRET peptides as substrates allowed the characterization of a repertoire of serine proteases and metalloproteases with different molecular weights secreted by Bacillus strains isolated from composting. Furthermore, thermostable serine and metalloproteases were detected in the composting leachate, which might be of interest for industrial applications. (AU)

FAPESP's process: 14/07037-0 - Chemical and biological assessment of composting produced at the Fundação Parque Zoológico de São Paulo (FPZSP).
Grantee:Luciana Teresa Dias Cappelini
Support Opportunities: Scholarships in Brazil - Post-Doctoral
FAPESP's process: 12/50191-4 - Synthesis, kinetic studies and applications of substrates and inhibitors for proteolytic enzymes
Grantee:Maria Aparecida Juliano
Support Opportunities: Research Projects - Thematic Grants
FAPESP's process: 13/12106-8 - Screening and characterization of serine carboxyl peptidases and glutamic peptidases in bacteria isolated from the composting operation at the São Paulo Zoo Park
Grantee:Márcia Yuri Kondo
Support Opportunities: Scholarships in Brazil - Post-Doctoral
FAPESP's process: 11/06548-2 - Polyphasic taxonomy of bacterial isolates from the composting of wastes from São Paulo Zoo
Grantee:Patrícia Locosque Ramos
Support Opportunities: Scholarships in Brazil - Post-Doctoral