Systems and Synthetic Biology Approaches to Engineer Fungi for Fine Chemical Production

Author(s)

Martins-Santana, Leonardo ^[1] ; Nora, Luisa C. ^[1] ; Sanches-Medeiros, Ananda ^[1] ; Lovate, Gabriel L. ^[1] ; Cassiano, Murilo H. A. ^[1] ; Silva-Rocha, Rafael ^[1]

Affiliation

^[1] Univ Sao Paulo, FMRP USP, Cell & Mol Biol Dept, Syst & Synthet Biol Lab, Ribeirao Preto Med Sch, Ribeirao Preto - Brazil

• Document type: Review article
• Source: FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY; v. 6, OCT 3 2018.
• Web of Science Citations: 5

Abstract

Since the advent of systems and synthetic biology, many studies have sought to harness microbes as cell factories through genetic and metabolic engineering approaches. Yeast and filamentous fungi have been successfully harnessed to produce fine and high value-added chemical products. In this review, we present some of the most promising advances from recent years in the use of fungi for this purpose, focusing on the manipulation of fungal strains using systems and synthetic biology tools to improve metabolic flow and the flow of secondary metabolites by pathway redesign. We also review the roles of bioinformatics analysis and predictions in synthetic circuits, highlighting in silico systemic approaches to improve the efficiency of synthetic modules. (AU)

• FAPESP's process: 15/22386-3 - Construction and characterization of constitutive mutant promoters and regulated by the global regulators CRP and Fis in Escherichia coli
• Grantee: Ananda Sanches Medeiros
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• FAPESP's process: 17/17924-1 - Construction of synthetic promoters for the expression of cellulases using novel cis-regulatory elements in Trichoderma reesei
• Grantee: Leonardo Martins Santana
• Support Opportunities: Scholarships in Brazil - Doctorate (Direct)
• FAPESP's process: 16/11093-8 - Construction of an ultrasensitive arsenic biosensor through synthetic biology approaches
• Grantee: Gabriel Lencioni Lovate
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• FAPESP's process: 17/04217-5 - Development of a computational tool for searching and strength prediction of bacterial promoters for applications in synthetic biology
• Grantee: Murilo Henrique Anzolini Cassiano
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• FAPESP's process: 16/03763-3 - Unveiling the mechanisms of gene regulation at the single cell level in Trichoderma reesei
• Grantee: Luísa Czamanski Nora
• Support Opportunities: Scholarships in Brazil - Master
• FAPESP's process: 12/22921-8 - Synthetic biology approaches for deciphering the logic of signal integration in complex bacterial promoters
• Grantee: Rafael Silva Rocha
• Support Opportunities: Research Grants - Young Investigators Grants