| Full text | |
| Author(s): |
Fábia Lima Vilarino
[1]
;
Bianca Bianco
[2]
;
Denise Maria Christofolini
[3]
;
Tatiana Goberstein Lerner
[4]
;
Caio Parente Barbosa
[5]
Total Authors: 5
|
| Affiliation: | [1] Faculdade de Medicina do ABC. Centro de Reprodução Humana e Genética. Programa de Pós-Graduação (Doutorado) - Brasil
[2] Faculdade de Medicina do ABC. Departamento de Ginecologia e Obstetrícia - Brasil
[3] Faculdade de Medicina do ABC. Departamento de Ginecologia e Obstetrícia - Brasil
[4] Faculdade de Medicina do ABC. Centro de Reprodução Humana e Genética - Brasil
[5] Faculdade de Medicina do ABC. Centro de Reprodução Humana e Genética - Brasil
Total Affiliations: 5
|
| Document type: | Journal article |
| Source: | Revista Brasileira de Ginecologia e Obstetrícia; v. 33, n. 2, p. 65-69, 2011-02-00. |
| Abstract | |
PURPOSE: to evaluate the frequency of VDR gene polymorphism Fok1 in infertile women with endometriosis and Control and its relation to the disease. METHODS: a case-control study that included 147 infertile women with endometriosis and 154 fertile women without endometriosis as Control. Fok1 polymorphism (rs10735810, T2C), which promotes a T/C exchange in exon 2 of the VDR gene, was identified by the polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP), that involves the combination of amplification by PCR and digestion with restriction endonuclease. The χ2 test was used to compare allele and genotype frequencies between groups. All p-values were two-tailed and a p-value < 0.05 was considered statistically significant. RESULTS: the TT, TC and CC genotype frequencies of VDR Fok1 polymorphism were 44.2%, 46.9% and 8.9% in infertile women with endometriosis and 41.6%, 50% and 8.4% in the Control Group. No significant difference was found (p=0.8), even when the patients were subdivided according to the stage of endometriosis (p=0.3 for minimal and mild endometriosis and p=0.2 for moderate and severe endometriosis). Alleles T and C were present, respectively, in 67.6% and 32.3% of infertile women with endometriosis (p=0.8), in 63.5% and 36.5% of women with minimal/mild endometriosis (p=0.5), in 72.5% and 27.5% of women with moderate/severe endometriosis (p=0.2), and in 66.6% and 33.4% of the Control Group. No statistically significant difference was found among any groups and the Control. CONCLUSION: the results suggest that VDR gene polymorphism Fok1 does not confer genetic susceptibility to endometriosis-associated infertility in the Brazilian population. (AU) | |
| FAPESP's process: | 10/01104-6 - Study of Fok1 polymorphism of VDR gene in women with endometriosis |
| Grantee: | Tatiana Goberstein Lerner |
| Support Opportunities: | Scholarships in Brazil - Scientific Initiation |
| FAPESP's process: | 10/00459-5 - Evaluation of VDR polymorphism in women with endometriosis |
| Grantee: | Caio Parente Barbosa |
| Support Opportunities: | Regular Research Grants |