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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

A Chemiresistor Sensor Based on Azo-Polymer and Graphene for Real-Time Monitoring of Mitochondrial Oxygen Consumption

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Author(s):
Olean-Oliveira, Andre [1] ; Olean-Oliveira, Tiago [2] ; Moreno, Ana C. R. [2] ; Seraphim, Patricia M. [2] ; Teixeira, Marcos F. S. [1]
Total Authors: 5
Affiliation:
[1] Sao Paulo State Univ UNESP, Dept Chem & Biochem, Sch Sci & Technol, Rua Roberto Simonsen 305, BR-19060900 Sao Paulo - Brazil
[2] Sao Paulo State Univ UNESP, Dept Physiotherapy, Sch Sci & Technol, Rua Roberto Simonsen 305, BR-19060900 Sao Paulo - Brazil
Total Affiliations: 2
Document type: Journal article
Source: ACS SENSORS; v. 4, n. 1, p. 118-125, JAN 2019.
Web of Science Citations: 0
Abstract

In the present study, a chemiresistor sensor based on a poly(Bismarck Brown Y)-reduced graphene oxide nanocomposite was developed to analyze the respiratory capacity of the constituent complexes of the electron transport chain. The sensorial platform was characterized using electrochemical impedance spectroscopy, and oxygen detection was accomplished by measuring the resistive properties of the sensor at fixed AC frequency. The impedance decreased significantly in response to small variations of the O-2 concentrations tested up to saturation of the electrolyte solution with molecular oxygen. The resistive response of the sensor at 0.1 Hz was linear over the oxygen concentration range from 1.17 X 10(-5) mol L-1 to 1.02 x 10(-3) mol L-1, with a detection limit of 3.60 X 10(-7) mol L-1. Using the new O-2 sensing platform, we monitored gradients in static cultures of adherent cells exposed to graded oxygen both at rest and upon metabolic stimulation. Under high dissolved oxygen conditions, the respiration of resting cells dictated that local O-2 was moderately reduced, while cell metabolic stimulation triggered a major redistribution of O-2. The usefulness of the developed sensor was demonstrated by continuous monitoring of mitochondrial oxygen consumption in various biologic applications. (AU)

FAPESP's process: 16/09017-1 - Study of the film formation of Schiff base metallic complexes
Grantee:Marcos Fernando de Souza Teixeira
Support type: Regular Research Grants
FAPESP's process: 13/07296-2 - CDMF - Center for the Development of Functional Materials
Grantee:Elson Longo da Silva
Support type: Research Grants - Research, Innovation and Dissemination Centers - RIDC