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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Biomimetic magnetic sensor for electrochemical determination of scombrotoxin in fish

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Hassan, Amal H. A. [1, 2] ; Sappia, Luciano [1] ; Lima Moura, Silio [1] ; Ali, Fatma H. M. [2] ; Moselhy, Walaa A. [3] ; Taboada Sotomayor, Maria del Pilar [4] ; Isabel Pividori, Maria [1]
Total Authors: 7
[1] Univ Autonoma Barcelona, Dept Quim, Grp Sensors & Biosensors, Bellaterra 08193 - Spain
[2] Beni Suef Univ, Fac Vet Med, Food Hyg & Control Dept, Bani Suwayf 62511 - Egypt
[3] Beni Suef Univ, Fac Vet Med, Forens Med & Toxicol Dept, Bani Suwayf 62511 - Egypt
[4] State Univ Sao Paulo UNESP, Inst Chem, Dept Analyt Chem, BR-14801970 Araraquara, SP - Brazil
Total Affiliations: 4
Document type: Journal article
Source: Talanta; v. 194, p. 997-1004, MAR 1 2019.
Web of Science Citations: 6

This work addresses a novel, rapid and cost-effective approach for the electrochemical sensing of scombrotoxin (histamine) in fish based on magnetic molecularly imprinted polymer (magnetic-MIP). The histamine magnetic-MIP was synthesized by the core-shell method using histamine as a template, and 2-vinyl pyridine as functional monomer. The magnetic-MIP was characterized by TEM, SEM, and confocal microscopy. Additionally, the binding capacity of magnetic-MIP towards histamine was investigated and compared with magnetic non-molecularly imprinted polymer (magnetic-NIP). This biomimetic material merged the advantages of MIPs and magnetic particles (MPs), including low cost of production, stability, high binding capacity and can be easily separated by the aid of a permanent magnet. The magnetic-MIP was integrated into magneto-actuated electrodes for the direct electrochemical detection of histamine preconcentrated from fish samples. The results revealed that this approach succeeded in the preconcentration and determination of histamine with a LOD as low as 1.6 x 10(-6) mg L-1, much lower than the index for fish spoilage (50 mg kg(-1)) accordingly to the legislation. Furthermore, the analytical performance was validated for the determination of histamine in scombroid fish samples with recovery values ranging from 96.8 to 102.0 %, confirm so it can be applied easily for routine food examination. (AU)

FAPESP's process: 14/50945-4 - INCT 2014: National Institute for Alternative Technologies of Detection, Toxicological Evaluation and Removal of Micropollutants and Radioactivies
Grantee:Maria Valnice Boldrin
Support type: Research Projects - Thematic Grants