Biomimetic magnetic sensor for electrochemical determination of scombrotoxin in fish

Author(s)

Hassan, Amal H. A. ^{[1, 2]} ; Sappia, Luciano ^[1] ; Lima Moura, Silio ^[1] ; Ali, Fatma H. M. ^[2] ; Moselhy, Walaa A. ^[3] ; Taboada Sotomayor, Maria del Pilar ^[4] ; Isabel Pividori, Maria ^[1]

Affiliation

^[1] Univ Autonoma Barcelona, Dept Quim, Grp Sensors & Biosensors, Bellaterra 08193 - Spain

^[2] Beni Suef Univ, Fac Vet Med, Food Hyg & Control Dept, Bani Suwayf 62511 - Egypt

^[3] Beni Suef Univ, Fac Vet Med, Forens Med & Toxicol Dept, Bani Suwayf 62511 - Egypt

^[4] State Univ Sao Paulo UNESP, Inst Chem, Dept Analyt Chem, BR-14801970 Araraquara, SP - Brazil

• Document type: Journal article
• Source: Talanta; v. 194, p. 997-1004, MAR 1 2019.
• Web of Science Citations: 6

Abstract

This work addresses a novel, rapid and cost-effective approach for the electrochemical sensing of scombrotoxin (histamine) in fish based on magnetic molecularly imprinted polymer (magnetic-MIP). The histamine magnetic-MIP was synthesized by the core-shell method using histamine as a template, and 2-vinyl pyridine as functional monomer. The magnetic-MIP was characterized by TEM, SEM, and confocal microscopy. Additionally, the binding capacity of magnetic-MIP towards histamine was investigated and compared with magnetic non-molecularly imprinted polymer (magnetic-NIP). This biomimetic material merged the advantages of MIPs and magnetic particles (MPs), including low cost of production, stability, high binding capacity and can be easily separated by the aid of a permanent magnet. The magnetic-MIP was integrated into magneto-actuated electrodes for the direct electrochemical detection of histamine preconcentrated from fish samples. The results revealed that this approach succeeded in the preconcentration and determination of histamine with a LOD as low as 1.6 x 10(-6) mg L-1, much lower than the index for fish spoilage (50 mg kg(-1)) accordingly to the legislation. Furthermore, the analytical performance was validated for the determination of histamine in scombroid fish samples with recovery values ranging from 96.8 to 102.0 %, confirm so it can be applied easily for routine food examination. (AU)

• FAPESP's process: 14/50945-4 - INCT 2014: National Institute for Alternative Technologies of Detection, Toxicological Evaluation and Removal of Micropollutants and Radioactivies
• Grantee: Maria Valnice Boldrin
• Support Opportunities: Research Projects - Thematic Grants