| Full text | |
| Author(s): |
Weslen Fabricio Pires Teixeira
[1]
;
Willian Marinho Dourado Coelho
[2]
;
Cáris Maroni Nunes
[3]
;
Marcelo Vasconcelos Meireles
[4]
Total Authors: 4
|
| Affiliation: | [1] Universidade Estadual Paulista. Faculdade de Medicina Veterinária
[2] Universidade Estadual Paulista. Faculdade de Ciências Agrárias e Veterinárias
[3] Universidade Estadual Paulista. Faculdade de Medicina Veterinária
[4] Universidade Estadual Paulista. Faculdade de Medicina Veterinária
Total Affiliations: 4
|
| Document type: | Journal article |
| Source: | Revista Brasileira de Parasitologia Veterinária; v. 20, n. 4, p. 269-273, 2011-12-00. |
| Abstract | |
The aim of this study was to produce a conjugate containing anti-Cryptosporidium parvum polyclonal antibodies and standardize a Direct Immunofluorescence Assay (DIF) for detecting C. parvum oocysts in fecal samples from calves. In order to obtain anti-C. parvum polyclonal antibodies, two New Zealand rabbits were immunized with a purified solution of C. parvum oocysts and Freund's adjuvant. Purification of the immunoglobulin G (IgG) fraction was performed by means of precipitation in ammonium sulfate and chromatography using a DEAE-cellulose column. The anti-C. parvum polyclonal antibody titer was determined by means of the enzyme-linked immunosorbent assay (ELISA). The rabbit anti-C. parvum IgG fraction was conjugated with fluorescein isothiocyanate and standardization of the DIF was performed using various dilutions of conjugate on slides positive for C. parvum oocysts. The cross-reactivity of the anti-C. parvum conjugate was tested using oocysts of Cryptosporidium serpentis, Cryptosporidium andersoni, Escherichia coli, Eimeria sp., and Candida sp. An anti-C. parvum conjugate was successfully produced, thus allowing standardization of DIF for detection of Cryptosporidium oocysts in fecal samples. Cross-reactivity of anti-C. parvum polyclonal antibodies with C. andersoni and C. serpentis was also observed. (AU) | |