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(Reference retrieved automatically from SciELO through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Staphylococcus aureus ampicillin-resistant from the odontological clinic environment

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Author(s):
Wagner Luis de Carvalho Bernardo [1] ; Marcelo Fabiano Gomes Boriollo [2] ; Reginaldo Bruno Gonçalves [3] ; José Francisco Höfling [4]
Total Authors: 4
Affiliation:
[1] Universidade Estadual de Campinas. Faculdade de Odontologia de Piracicaba. Laboratório de Microbiologia e Imunologia - Brasil
[2] Universidade Estadual de Campinas. Faculdade de Odontologia de Piracicaba. Laboratório de Microbiologia e Imunologia - Brasil
[3] Universidade Estadual de Campinas. Faculdade de Odontologia de Piracicaba. Laboratório de Microbiologia e Imunologia - Brasil
[4] Universidade Estadual de Campinas. Faculdade de Odontologia de Piracicaba. Laboratório de Microbiologia e Imunologia - Brasil
Total Affiliations: 4
Document type: Journal article
Source: Revista do Instituto de Medicina Tropical de São Paulo; v. 47, n. 1, p. 19-24, 2005-02-00.
Abstract

The aim of this research was to evaluate the prevalence of Sthaphylococcus spp. and S. aureus in the odontological clinic environment (air), their production of beta-lactamase and antibacterial susceptibility to the major antibiotics utilized in medical particle. During 12 months of samples collect were isolated 9775 CFU by MSA medium suggesting a high amount of Staphylococcus spp. in the clinic environment which can appear through aerosols. A total of 3149 colonies (32.2%) were suggestive of pathogenic staphylococci. Gram coloration, catalase test, colony-mallow growing on chromogenic medium, and coagulase test confirmed the identity of 44 (0.45%) S. aureus isolates. Of these, 35 isolates (79.5%) showed production of beta-lactamase by CefinaseTM discs and resistance to ampicillin, erythromycin (7 isolates) and tetracycline (1 isolate) suggesting the existence of multiresistant isolates. The evaluation of the oxacillin MIC by Etest® assays showed susceptibility patterns suggesting the inexistence of the mecA gene in chromosomal DNA. These results point out to the need of a larger knowledge on the contamination means and propagation of this microorganism into the odontological clinic. (AU)