| Full text | |
| Author(s): |
Silene Peres Keusseyan
[1]
;
Neusa Pereira da Silva
[2]
;
Maria Odete Esteves Hilário
[3]
;
Eunice Mitiko Okuda
[4]
;
Maria Teresa S. L. R. Ascenção Terreri
;
Luis Eduardo Coelho Andrade
[6]
Total Authors: 6
|
| Affiliation: | [1] Universidade Federal de São Paulo
[2] Universidade Federal de São Paulo
[3] Universidade Federal de São Paulo
[4] Santa Casa de Misericórdia de São Paulo. Faculdade de Ciências Médicas
[6] Universidade Federal de São Paulo
Total Affiliations: 6
|
| Document type: | Journal article |
| Source: | REVISTA BRASILEIRA DE REUMATOLOGIA; v. 52, n. 5, p. 703-712, 2012-10-00. |
| Abstract | |
OBJECTIVES: To determine the frequency of antibodies to chromatin components in juvenile systemic lupus erythematosus (JSLE), and to correlate the presence of these autoantibodies with clinical manifestations and disease activity. METHODS: Anti-chromatin (anti-CHR), anti-nucleosome core particle (anti-NCS) and anti-dsDNA antibodies were measured in 175 individuals, including 37 patients with active JSLE and 41 with inactive disease, 47 non-lupus autoimmune disease patients (non-lupus AD), and 50 healthy children. An in-house ELISA was developed with purified nucleosome core particles from calf thymus to determine IgG and IgG3 anti-NCS antibodies. Anti-CHR and anti-dsDNA antibodies were detected by commercial ELISA kits (INOVA). RESULTS: Anti-NCS and anti-CHR antibodies exhibited high specificity for JSLE and similar frequency in active and inactive JSLE. Anti-CHR and IgG/IgG3 anti-NCS serum levels did not differ between active and inactive JSLE. SLEDAI correlated with anti-dsDNA antibodies but not with antibodies to other chromatin components. There was association of anti-dsDNA, anti-CHR and IgG/IgG3 anti-NCS antibodies with proteinuria and low C4 serum levels. Anti-NCS antibodies in the absence of anti-dsDNA were observed in 14% of the JSLE patients. CONCLUSIONS: Our data indicate that anti-NCS and anti-CHR antibodies are relevant diagnostic markers for JSLE and appear to be correlated with JSLE lupus nephritis activity. IgG3 isotype anti-NCS antibodies do not seem to be more relevant than IgG anti-NCS antibodies as markers of disease activity or active nephritis in JSLE. (AU) | |
| FAPESP's process: | 05/00658-0 - Diagnostic performace and clinical associations of antinucleosome antibodies in juvenile systemic lupus erythematosus |
| Grantee: | Luiz Eduardo Coelho Andrade |
| Support Opportunities: | Regular Research Grants |