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(Reference retrieved automatically from SciELO through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

IgM-ELISA for diagnosis of schistosomiasis mansoni in low endemic areas

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Author(s):
Edward José de Oliveira [1] ; Hermínia Yoshio Kanamura [2] ; Luiz Cândido de Souza Dias [3] ; Lanny Cristina Burlandy Soares [4] ; Dirce Mary Correia Lima [5] ; Ricardo Mário de Carvalho Ciaravolho [6]
Total Authors: 6
Affiliation:
[1] Universidade de São Paulo. Faculdade de Ciências Farmacêuticas. Departamento de Análises Clínicas e Toxicológicas - Brasil
[2] Universidade de São Paulo. Faculdade de Ciências Farmacêuticas. Departamento de Análises Clínicas e Toxicológicas - Brasil
[3] Universidade Estadual de Campinas. Faculdade de Ciências Médicas. Departamento de Patologia Clínica - Brasil
[4] Universidade Estadual de Campinas. Faculdade de Ciências Médicas. Departamento de Patologia Clínica - Brasil
[5] Universidade de São Paulo. Faculdade de Ciências Médicas. Instituto de Medicina Tropical de São Paulo - Brasil
[6] Secretaria de Saúde do Estado de São Paulo. Superintendência de Controle de Endemias - Brasil
Total Affiliations: 6
Document type: Journal article
Source: Cadernos de Saúde Pública; v. 19, n. 1, p. 255-261, 2003-02-00.
Abstract

An immunoenzymatic method for the detection of IgM antibodies (IgM-ELISA) against a fraction of Schistosoma mansoni adult worm antigen, soluble in trichloroacetic acid (TCA-soluble fraction), was evaluated for epidemiological purposes in low endemic areas for schistosomiasis. Blood samples on filter paper were collected from a population living in the municipality of Pedro de Toledo, São Paulo State, and submitted to IgM-ELISA. The results were compared to those obtained by the IgM-immunofluorescence test (IgM-IFT) and the Kato-Katz parasitological method. Positive rates of 36.8%, 33.5%, and 1.6% were obtained respectively by the IgM-ELISA, IgM-IFT, and Kato-Katz methods. The geometric mean obtained by the parasitological method was 40.9 eggs per gram of feces (epg). The nearly perfect agreement observed between the two serological tests (Kappa index of 0,89) indicates good diagnostic performance by the evaluated test. IgM-ELISA is a potentially useful method for diagnosis of schistosomiasis in individuals with low worm burden. (AU)