| Full text | |
| Author(s): |
Ferreira Lima, Ana Mara
[1]
;
Daniel, Camila Ribeiro
[2]
;
Navarro, Ricardo Scarparo
[3]
;
Bodanese, Benito
[4]
;
Pasqualucci, Carlos Augusto
[5]
;
Tavares Pacheco, Marcos Tadeu
[2]
;
Zangaro, Renato Amaro
[2]
;
Silveira Jr, Landulfo
Total Authors: 8
|
| Affiliation: | [1] Christus Fac Piaui CHRISFAPI, Rua Acelino Rezende 132, BR-64260000 Piripiri, PI - Brazil
[2] UAM, CITE, Parque Tecnol Sao Jose dos Campos, Estr Dr Altino Bondensan 500, BR-12247016 Sao Jose Dos Campos, SP - Brazil
[3] Univ Brasil, Rua Carolina Fonseca 584, BR-08230030 Sao Paulo, SP - Brazil
[4] HRO, Dept Oncol, R Florianopolis 1448-E, BR-89812021 Chapeco, SC - Brazil
[5] Univ Sao Paulo, Med Sch, Dept Pathol, FMUSP, LIM 22, Av Dr Arnaldo 455, BR-01246903 Sao Paulo, SP - Brazil
Total Affiliations: 5
|
| Document type: | Journal article |
| Source: | VIBRATIONAL SPECTROSCOPY; v. 100, p. 131-141, JAN 2019. |
| Web of Science Citations: | 4 |
| Abstract | |
This study aimed the diagnosis of non-melanoma skin cancer (basal cell carcinoma - BCC, squamous cell carcinoma - SCC) and actinic keratosis (AK) and normal tissue (NO) by means of Raman spectra collected in vivo and ex vivo as well as determine, through the main Raman features, which biochemical element present in the skin is related to the spectral changes in the lesions compared to normal tissue. A total of 471 Raman spectra in vivo and 227 spectra ex vivo from NO, BCC, SCC and AK tissues were collected using a dispersive Raman spectrometer (830 nm excitation, 30 s exposure time, 200 mW laser' power) as well as spectra of basal skin constituents (proteins, lipids, nucleic acids). Student's t-test (p < 0.01) was applied to identify the Raman peaks with significant differences of lesion compared to normal skin and discriminant analysis based on Euclidian and Mahalanobis distances were applied to the intensities of the significant peaks. The results showed better discrimination between lesions and normal tissue through Mahalanobis distance both in vivo and ex vivo, with sensitivity, specificity and overall accuracy of 94.1%, 93.6% and 93.8%, respectively, for the in vivo spectra, while the discrimination showed sensitivity, specificity and overall accuracy of 100% for ex vivo tissues. The use of the intensities of the significant Raman peaks in the discrimination model showed results compared to other multivariate methods and may allow faster processing time, increasing the possibility of clinical use. (AU) | |
| FAPESP's process: | 09/01788-5 - Dispersive Raman spectroscopy using optical fibers "Raman probe" applied to the diagnosis of neoplasia in the skin and prostate |
| Grantee: | Landulfo Silveira Junior |
| Support Opportunities: | Regular Research Grants |