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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Synapse preservation and decreased glial reactions following ventral root crush (VRC) and treatment with 4-hydroxy-tempo (TEMPOL)

Full text
Author(s):
Spejo, Aline Barroso [1] ; Teles, Caroline Brandao [1] ; Zuccoli, Giuliana da Silva [1] ; Rodrigues de Oliveira, Alexandre Leite [1]
Total Authors: 4
Affiliation:
[1] Univ Campinas UNICAMP, Inst Biol, Dept Struct & Funct Biol, Campinas, SP - Brazil
Total Affiliations: 1
Document type: Journal article
Source: Journal of Neuroscience Research; v. 97, n. 4, p. 520-534, APR 2019.
Web of Science Citations: 0
Abstract

Astrogliosis and microglial reactions are correlated with the formation of scar tissue and synapse loss. 4-hydroxy-tempo (TEMPOL) is a reactive oxygen species scavenger with proven neuroprotective efficacy in experimental models of traumatic injury and cerebral ischemia. TEMPOL has not, however, been applied following ventral root lesions, which are particularly correlated with the degeneration of spinal motoneurons following brachial plexus injuries. Thus, the present study investigated the effects of TEMPOL on motoneurons and adjacent glial reactions, with a particular focus on the preservation of excitatory and inhibitory spinal circuits. Adult female Sprague Dawley rats were subjected to ventral root crush (VRC) at the lumbar intumescence. Animals were divided into the following experimental groups: (a) VRC-saline treatment; (b) VRC-TEMPOL treatment (12 mg/kg, n = 5), and (c) VRC-TEMPOL treatment (250 mg/kg, n = 5). The spinal cord tissue located contralateral to the lesion was used as the control. Fourteen days after lesioning, the rats were euthanized and the spinal cords were removed for motoneuron counting and immunolabeling with glial (GFAP and Iba-1) and synapse markers (synaptophysin, VGLUT-1, and GAD65). Although TEMPOL did not exert neuroprotective effects at the studied concentrations, the modulation of glial reactions was significant at higher doses. Thus, synaptophysin staining was preserved and, in particular, VGLUT-1-positive inputs were maintained, thereby indicating that TEMPOL preserved proprioceptive glutamatergic inputs without exacerbating the rate of motoneuron degeneration. Consequently, its administration with other efficient neuroprotective substances may significantly improve the outcomes following spinal cord lesioning. (AU)

FAPESP's process: 14/12707-4 - Neuroprotective effect of tempol (4-hidroxi tempo) after axonotmesis of motor roots in the CNS and PNS interface
Grantee:Giuliana da Silva Zuccoli
Support Opportunities: Scholarships in Brazil - Scientific Initiation
FAPESP's process: 12/22750-9 - Use of fibrin sealant combined with mesenchymal stem cells for anterior funiculus injury repair: effects on inflammation, axonal regeneration and neuroprotection.
Grantee:Aline Barroso Spejo
Support Opportunities: Scholarships in Brazil - Doctorate
FAPESP's process: 14/12671-0 - Effect of tempol (4-hydroxy-tempo) on synaptic plasticity, regeneration and inflammation after the crushing of ventral roots in the CNS and PNS interface
Grantee:Caroline Brandão Teles Rodrigues
Support Opportunities: Scholarships in Brazil - Scientific Initiation
FAPESP's process: 14/06892-3 - Use of mesenchymal stem cells in the CNS/PNS interface: repair of proximal lesions
Grantee:Alexandre Leite Rodrigues de Oliveira
Support Opportunities: Research Projects - Thematic Grants