| Full text | |
| Author(s): |
Alves, Glaucia Pereira
[1]
;
Bertuccez Cordeiro, Fernanda
[2]
;
de Lima, Camila Bruna
[1]
;
Annes, Kelly
[1]
;
dos Santos, Erika Cristina
[1]
;
Ispada, Jessica
[1]
;
Fontes, Patricia Kubo
[3]
;
Gouveia Nogueira, Marcelo Fabio
[4]
;
Nichi, Marcilio
[5]
;
Milazzotto, Marcella Pecora
[1]
Total Authors: 10
|
| Affiliation: | [1] Fed Univ ABC, Ctr Nat Sci & Humanities, Lab Cellular & Mol Biol, Av Estados 5005, BR-09210190 Santo Andre, SP - Brazil
[2] ESPOL, Escuela Super Politecn Litoral, Lab Invest Biomed, Guayaquil - Ecuador
[3] Univ Estadual Paulista UNESP, Inst Biosci, Campus Botucatu, Sao Paulo - Brazil
[4] Univ Estadual Paulista UNESP, Sch Sci & Languages, Dept Biol Sci, Campus Assis, Assis, SP - Brazil
[5] Univ Sao Paulo, Fac Vet Med, Dept Anim Reprod, Sao Paulo, SP - Brazil
Total Affiliations: 5
|
| Document type: | Journal article |
| Source: | REPRODUCTION FERTILITY AND DEVELOPMENT; v. 31, n. 3, p. 451-461, FEB 2019. |
| Web of Science Citations: | 0 |
| Abstract | |
Follicular fluid composition and the transcription pattern of granulosa cells were analysed to better comprehend associations between embryo development and morphokinetics. Bovine follicles were punctured and their respective follicular fluid and granulosa cells were collected. Cumulus-oocyte complexes derived from these follicles were matured and fertilised invitro. Embryo morphology and kinetics were evaluated at 40h after insemination, when embryos were classified as fast (FCL, four or more cells), slow (SCL, 2-3 cells) or non-cleaved (NCL). Their development was followed until the blastocyst stage. Glucose, pyruvate, cholesterol and oestradiol were quantified in the follicular fluid and the transcription pattern of 96 target genes was evaluated in granulosa cells by large-scale quantitative reverse transcription polymerase chain reaction. Follicular fluid from the blastocyst group had increased levels of glucose, total cholesterol and pyruvate compared to the non-blastocyst group, whereas higher levels of oestradiol were observed in the follicular fluid of embryos and blastocysts with fast cleavage. The transcriptional pattern revealed altered metabolic pathways between groups, such as lipid metabolism, cellular stress and cell signalling. In conclusion, both follicular fluid and granulosa cells are associated with the possibility of identifying follicles that may generate embryos with high potential to properly develop to the blastocyst stage. (AU) | |
| FAPESP's process: | 12/10351-2 - Metabolomics for non-invasive evaluation of in vitro produced bovine embryos |
| Grantee: | Marcella Pecora Milazzotto |
| Support Opportunities: | Regular Research Grants |
| FAPESP's process: | 12/50533-2 - GIFT: genomic improvement of fertilization traits in Danish and Brazilian cattle |
| Grantee: | Marcelo Fábio Gouveia Nogueira |
| Support Opportunities: | Research Projects - Thematic Grants |
| FAPESP's process: | 15/03381-0 - CELLULAR STRESS AND ITS RELATION WITH THE KINECTICS OF IN VITRO PRODUCED BOVINE EMBRYOS |
| Grantee: | Marcella Pecora Milazzotto |
| Support Opportunities: | Regular Research Grants |