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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Comparison of the Malpighian tubules and fat body transcriptional profiles of Zophobas morio larvae (Coleoptera: Tenebrionidae)

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Author(s):
Silva, Jaqueline R. [1, 2] ; Amaral, Danilo T. [1] ; Viviani, Vadim R. [1, 2]
Total Authors: 3
Affiliation:
[1] Univ Fed Sao Carlos, Grad Sch Biotechnol & Environm Monitoring, Sorocaba, SP - Brazil
[2] Univ Fed Sao Carlos, Grad Sch Evolut Genet & Mol Biol, Sao Carlos, SP - Brazil
Total Affiliations: 2
Document type: Journal article
Source: Comparative Biochemistry and Physiology D-Genomics & Proteomics; v. 29, p. 95-105, MAR 2019.
Web of Science Citations: 2
Abstract

The Malpighian tubules in insects play an essential role in osmoregulation, through the transport of ions during excretion, whereas the fat body is usually associated with the intermediary metabolism. The tubules also are involved in excretion of organic solutes and xenobiotics. However, with the exception of a preliminary transcriptional survey of the Zophobas morio (Tenebrionidae) larval tubules, there are no detailed transcriptional analysis of this organ in Coleoptera. A luciferase-like enzyme that displays weak luminescence activity in the presence of firefly D-luciferin and ATP was cloned from the tubules of Z. morio larvae. In order to better understand the molecular physiology of Malpighian tubules and fat body in Coleoptera larvae, and to investigate the occurrence and functions of AMP-CoA ligases in these tissues, we performed a comparative transcriptional analysis of these tissues using Z. morio giant-mealworms. As expected, the tubules displayed organic and inorganic transporters, xenobiotic metabolism enzymes, V-ATPases, channels, and pumps. The fat body showed proteins that are synthesized in this tissue and secreted to the hemolymph, as well as enzymes involved in lipid and carbohydrate metabolism. These tissues are also involved in common pathways, such as nitrogen metabolism to degradation/excretion, eye pigments biosynthesis, immunity, and detoxification. The presence of coumarate-CoA ligase-like enzymes in these tissues suggest their involvement in the degradation of coumaric acid derivatives obtained from the diet, or alternatively, in the biosynthesis of compounds structurally related to coumaric acids such as eye pigments. Our results confirm to the physiological versatility of tubules and fat body in larval Coleoptera. (AU)

FAPESP's process: 10/05426-8 - Arthropod bioluminescence: biological diversity in Brazilian biomes, biochemical origin, structural/functional evolution of luciferases, molecular differentiation of lanterns, biotechnological, environmental and educational applications
Grantee:Vadim Viviani
Support Opportunities: Research Projects - Thematic Grants
FAPESP's process: 13/09594-0 - Analysis of coleopteran lanterns, fat body and Malpighian tubules transcriptomes, and its relationship with the origins of bioluminescence in Coleoptera and Diptera
Grantee:Vadim Viviani
Support Opportunities: Regular Research Grants
FAPESP's process: 12/25378-3 - Transcriptome and AMP-ligases function in Malpighian tubules physiology of Zophobas morio larvae (Coleoptera: Tenebrionidae)
Grantee:Jaqueline Rodrigues da Silva
Support Opportunities: Scholarships in Brazil - Doctorate (Direct)