Advanced search
Start date
Betweenand

Analysis of coleopteran lanterns, fat body and Malpighian tubules transcriptomes, and its relationship with the origins of bioluminescence in Coleoptera and Diptera

Abstract

Bioluminescence in insects is found mainly in Coleoptera and Diptera. In Beetles of the superfamily Elateroidea, bioluminescence involves the same bioluminescence system with luciferins and luciferases well characterized. Beetle luciferin is a benzothiazolic compound, however, its biosynthetic origin remains unknown. The lanterns of bioluminescent beetles probably evolved from extensions of the fat body. In Arachnocampa spp (Diptera: Mycetophylidae), the bioluminescent system remains poorly known, involving lanterns arising from extensions of the Malpighian tubules, an unknown luciferin, an unidentified luciferase and ATP. Although the bioluminescent systems of beetles and Arachnocampa are not evolutionarily related, the involvement of ATP in both systems suggests that Arachnocampa may also employ an AMP-ligase as a luciferase. This hypothesis is supported by recent finding of luciferase-like enzymes in the Malpighian tubules of Zophobas morio (Coleoptera:Tenebrionidae) larvae. Considering that the biosynthetic origin of beetle luciferin and the molecular physiology of the lanterns are poorly known, we aim to investigate the transcriptomes of the adult and larval lanterns, and fat body of Aspisoma lineatum firefly (Coleoptera:Lampyridae), to identify potential enzymatic targets involved with the above processes. Also, considering that no transcriptome of the Malpíghian tubules is currently known for any Coleoptera, and the existence of luciferase-like enzymes of unknown function in the Malpighian tubules of tenebrionid beetles, suggesting a possible evolutive link between AMP-ligases and the origin of Arachnocampa lanterns, we aim to investigate the transcriptome of these organs in Zophobas morio (Coleoptera:Tenebrionidae) larvae comparing with the transcriptome of Arachnocampa flava (Diptera:Mycetophylidae) lanterns. (AU)

Articles published in Agência FAPESP Newsletter about the research grant:
Articles published in other media outlets (0 total):
More itemsLess items
VEICULO: TITULO (DATA)
VEICULO: TITULO (DATA)

Scientific publications (11)
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
AMARAL, DANILO T.; SILVA, JAQUELINE R.; VIVIANI, VADIM R. RNA-Seq analysis of the bioluminescent and non-bioluminescent species of Elateridae (Coleoptera): Comparison to others photogenic and non-photogenic tissues of Elateroidea species. Comparative Biochemistry and Physiology D-Genomics & Proteomics, v. 29, p. 154-165, MAR 2019. Web of Science Citations: 1.
SILVA, JAQUELINE R.; AMARAL, DANILO T.; VIVIANI, VADIM R. Comparison of the Malpighian tubules and fat body transcriptional profiles of Zophobas morio larvae (Coleoptera: Tenebrionidae). Comparative Biochemistry and Physiology D-Genomics & Proteomics, v. 29, p. 95-105, MAR 2019. Web of Science Citations: 2.
VIVIANI, VADIM R.; GABRIEL, GABRIELE V. M.; BEVILAQUA, VANESSA R.; SIMOES, A. F.; HIRANO, T.; LOPES-DE-OLIVEIRA, P. S. The proton and metal binding sites responsible for the pH-dependent green-red bioluminescence color tuning in firefly luciferases. SCIENTIFIC REPORTS, v. 8, DEC 4 2018. Web of Science Citations: 4.
AMARAL, DANILO T.; MITANI, YASUO; OLIVEIRA, GABRIELA; OHMIYA, YOSHIHIRO; VIVIANI, VADIM R. Revisiting Coleoptera a plus T-rich region: structural conservation, phylogenetic and phylogeographic approaches in mitochondrial control region of bioluminescent Elateridae species (Coleoptera). MITOCHONDRIAL DNA PART A, v. 28, n. 4-5, p. 671-680, SEP 2017. Web of Science Citations: 4.
PEREZ, ARINA MARINA; AQUINO, BRUNO; VIVIANI, VADIM; KOBARG, JORG. Use of a special Brazilian red-light emitting railroad worm Luciferase in bioassays of NEK7 protein Kinase and Creatine Kinase. BMC BIOCHEMISTRY, v. 18, JUL 19 2017. Web of Science Citations: 0.
GABRIEL, GABRIELE V. M.; VIVIANI, VADIM R. Engineering the metal sensitive sites in Macrolampis sp2 firefly luciferase and use as a novel bioluminescent ratiometric biosensor for heavy metals. ANALYTICAL AND BIOANALYTICAL CHEMISTRY, v. 408, n. 30, p. 8881-8893, DEC 2016. Web of Science Citations: 2.
VIVIANI, V. R.; SIMOES, A.; BEVILAQUA, V. R.; GABRIEL, G. V. M.; ARNOLDI, F. G. C.; HIRANO, T. Glu311 and Arg337 Stabilize a Closed Active-site Conformation and Provide a Critical Catalytic Base and Countercation for Green Bioluminescence in Beetle Luciferases. BIOCHEMISTRY, v. 55, n. 34, p. 4764-4776, AUG 30 2016. Web of Science Citations: 7.
AMARAL, DANILO T.; MITANI, YASUO; OHMIYA, YOSHIHIRO; VIVIANI, VADIM R. Organization and comparative analysis of the mitochondrial genomes of bioluminescent Elateroidea (Coleoptera: Polyphaga). Gene, v. 586, n. 2, p. 254-262, JUL 25 2016. Web of Science Citations: 10.
AMARAL, DANILO T.; OLIVEIRA, GABRIELA; SILVA, JAQUELINE R.; VIVIANI, VADIM R. A new orange emitting luciferase from the Southern-Amazon Pyrophorus angustus (Coleoptera: Elateridae) click-beetle: structure and bioluminescence color relationship, evolutional and ecological considerations. PHOTOCHEMICAL & PHOTOBIOLOGICAL SCIENCES, v. 15, n. 9, p. 1148-1154, 2016. Web of Science Citations: 4.

Please report errors in scientific publications list by writing to: cdi@fapesp.br.
Filed patent(s) as a result of this research project

Solicitação em análise e dentro do prazo legal de sigilo previsto na legislação BR102017023885-7 - Universidade Estadual de Campinas (UNICAMP) . Solicitação em análise e dentro do prazo legal de sigilo previsto na legislação - November 2017, 06