Molecular epidemiology of methicillin-resistant Staphylococcus aureus in the Brazilian primary health care system

Author(s)

Lino Pereira-Franchi, Eliane Patricia ^[1] ; Nogueira Barreira, Maria Rachel ^[1] ; Lima Moreira da Costa, Natalia de Sousa ^[1] ; Moraes Riboli, Danilo Flavio ^[1] ; Abraao, Ligia Maria ^{[1, 2]} ; Martins, Katheryne Benini ^[1] ; Victoria, Cassiano ^[3] ; Ribeiro de Souza da Cunha, Maria de Lourdes ^[1]

Affiliation

^[1] Univ Estadual Paulista Julio de Mesquita Filho UN, Inst Biociencias Botucatu, Dept Microbiol & Imunol, Botucatu, SP - Brazil

^[2] Univ Estadual Paulista Julio de Mesquita Filho UN, Fac Med Botucatu, Dept Doencas Tropicais, Botucatu, SP - Brazil

^[3] Univ Estadual Paulista Julio de Mesquita Filho UN, Fac Med Vet & Zootecnia, Dept Higiene Vet & Saude Publ, Botucatu, SP - Brazil

• Document type: Journal article
• Source: TROPICAL MEDICINE & INTERNATIONAL HEALTH; v. 24, n. 3, p. 339-347, MAR 2019.
• Web of Science Citations: 0

Abstract

Objective To evaluate the molecular epidemiology and to georeference Staphylococcus aureus isolated from wounds and nares of patients seen at Basic Health Units (BHUs) of a Brazilian city. Methods Observational, cross-sectional study conducted from 2010 to 2013. A total of 119 S. aureus strains isolated from the wounds and nares of 88 patients were studied. The isolates were characterised by identifying virulence genes encoding enterotoxins A-E, haemolysins alpha, beta and delta, exfoliatins A, B and D, biofilm production, Panton-Valentine Leukocidin and toxic shock syndrome toxin 1, and by pulsed-field gel electrophoresis (PFGE), multilocus sequence and spa typing. Results Eighteen methicillin-resistant Staphylococcus aureus (MRSA) (6 SCCmec type II and 12 SCCmec type IV) and 101 (85%) MSSA were identified. PFGE typing resulted in the formation of eight clusters, with STs 1, 5, 8, 30, 188, 1176 and 1635 and spa type t002 being the predominant types among MSSA. The 18 MRSA belonged to STs 5, 8 and 1176 and spa types t002 and t062. Conclusion The results demonstrate widespread dissemination of MSSA and MRSA clones carrying haemolysin, biofilm and toxin genes. Kernel density estimation revealed the highest density of S. aureus in the 4, 5 and 8 BHUs. (AU)

• FAPESP's process: 12/00257-9 - Molecular epidemiology and study of virulence factors of Staphylococcus aureus resistant to oxacillin isolated from wounds in patients treated at Basic Health Units in the city of Botucatu
• Grantee: Eliane Patricia Lino Pereira Franchi
• Support Opportunities: Scholarships in Brazil - Doctorate
• FAPESP's process: 13/10975-9 - Protective efficacy of active immunization with the recombinant lipoproteins of Staphylococcus aureus in an experimental model of bacteremia
• Grantee: Eliane Patricia Lino Pereira Franchi
• Support Opportunities: Scholarships abroad - Research Internship - Doctorate
• FAPESP's process: 11/10146-7 - Molecular epidemiology and study of the virulence factors of Staphylococcus aureus methicillin resistant isolated from wounds in patients treated in Basic Health Units in the city of Botucatu
• Grantee: Maria de Lourdes Ribeiro de Souza da Cunha
• Support Opportunities: Regular Research Grants