Physicochemical, morphological, and biological analyses of Ti-15Mo alloy surface modified by laser beam irradiation

Author(s)

Pires, Luana C. ^[1] ; Guastaldi, Fernando P. S. ^[1] ; Nogueira, Andressa V. B. ^[1] ; Oliveira, Nilson T. C. ^[2] ; Guastaldi, Antonio C. ^[3] ; Cirelli, Joni A. ^{[1, 4]}

Affiliation

^[1] Sao Paulo State Univ UNESP, Sch Dent Araraquara, Dept Diag & Surg, Araraquara, SP - Brazil

^[2] Univ Sao Paulo, Inst Chem Sao Carlos, Interfacial Electrochem Lab, Sao Carlos, SP - Brazil

^[3] UNESP, Inst Chem Araraquara, Dept Phys Chem, Araraquara, SP - Brazil

^[4] UNESP, Fac Odontol Araraquara, Dept Diagnost & Cirurgia, Rua Humaita 1680, BR-14801903 Araraquara, SP - Brazil

• Document type: Journal article
• Source: Lasers in Medical Science; v. 34, n. 3, p. 537-546, APR 2019.
• Web of Science Citations: 0

Abstract

Perform a physicochemical and morphological characterization of a Ti-15Mo alloy surface modified by laser beam irradiation and to evaluate in vitro the morphological response and proliferation of osteoblastic cells seeded onto this alloy. Disks were made of two different metals, Ti-15Mo alloy and cpTi, used as control. A total of four groups were evaluated: polished cpTi (cpTi-pol), laser-irradiated cpTi (cpTi-L), polished Ti-15Mo alloy (Ti-15Mo-pol), and laser-irradiated Ti-15Mo alloy (Ti-15Mo-L). Before and after laser irradiation of the surfaces, physicochemical and morphological analyses were performed: scanning electron microscopy (FEG-SEM), energy-dispersive spectroscopy (EDX), and X-ray diffraction (XRD). The wettability of the samples was evaluated by contact angle measurement. Murine preosteoblastic cells MC3T3-E1 were cultured onto the experimental disks for cell proliferation, morphology, and spreading analyses. Laser groups presented irregular-shaped cavities on its surface and a typical microstructured surface with large depressions (FEG-SEM). The contact angle for both laser groups was 0 degrees, whereas for the polished groups was approximate to 77 and approximate to 78 for cpTi-pol and Ti-15Mo-pol, respectively. Cell proliferation analysis demonstrated a higher metabolic activity in the laser groups (p<0.05). From the fluorescence microscopy, Ti-15Mo-L surface seems to induce greater cellular differentiation compared to the cpTi-L surface. The preliminary biological in vitro analyses suggested possible advantages of laser surface treatment in the Ti-15Mo alloy regarding cell proliferation and maturation. (AU)

• FAPESP's process: 12/17226-9 - In vitro behavior of bone cells on titanium surfaces with nanoscale topography, modified by laser with or without deposition of apatites
• Grantee: Joni Augusto Cirelli
• Support Opportunities: Regular Research Grants