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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Poly (lactic-co-glycolic acid) nanospheres allow for high L-asparaginase encapsulation yield and activity

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Author(s):
Medeiros de Brito, Anna Emmanuela [1] ; Pessoa, Jr., Adalberto [2] ; Converti, Attilio [3] ; Rangel-Yagui, Carlota de Oliveira [2] ; da Silva, Jose Alexsandro [4] ; Apolinario, Alexsandra Conceicao [2]
Total Authors: 6
Affiliation:
[1] State Univ Paraiba, Grad Program Pharmaceut Sci, Rua Juvencio Arruda S-N, BR-58429600 Campina Grande, Paraiba - Brazil
[2] Univ Sao Paulo, Dept Pharmaceut & Biochem Technol, Av Prof Linen Prestes 580, Bloco 16, Cidade Univ, BR-05508000 Sao Paulo - Brazil
[3] Genoa Univ, Dept Civil Chem & Environm Engn, Chem Engn Pole, Via Opera Pia 15, I-16145 Genoa - Italy
[4] Univ Fed Campina Grande, Dept Agrarian & Exact Sci, Postgrad Program Agroind Syst, Campus 4 UEPB, Pombal, Paraiba - Brazil
Total Affiliations: 4
Document type: Journal article
Source: Materials Science & Engineering C-Materials for Biological Applications; v. 98, p. 524-534, MAY 2019.
Web of Science Citations: 0
Abstract

L-Asparaginase (ASNase) is an amidohydrolase used as a chemotherapeutic agent for the treatment of acute lymphoblastic leukemia (ALL). The nanoencapsulation of this enzyme is strategic to avoid its immediate immunogenic effects that lead to a decrease in the enzyme half-life. In this work, ASNase-containing nanoparticles (NPs) were prepared by double emulsification, through an ultrasonic sonicator or an Ultra-Turrax, using two copolymers of 50:50 (w/w) poly (lactic-co-glycolic acid) (PLGA) with different ranges of molecular weight (24-38 kDa and 30-60 kDa) and varying the concentration of polyvinyl alcohol (PVA) as a stabilizer (0.5, 1.0, 1.5 and 2.0%) as well as the emulsification time (30 and 60 s). Using 24-38 kDa PLGA and 1.0% PVA, we obtained by cavitation NPs with hydrodynamic diameter of 384 nm, polydispersity index of 0.143 and Zeta potential of -16.4 mV, whose ASNase encapsulation efficiency was as high as 87 +/- 2%. The encapsulated enzyme showed an activity 22% higher than that of the free enzyme, and no conformational changes were detected by circular dichroism. The enzyme release from NPs entrapped in dialysis bags (500 kDa molecular weight cut-off) allowed selecting a controlled system able to release about 60% of the enzyme within 14 days, for which the Korsmeyer-Peppas model provided the best correlation (R-2 = 0.966). (AU)

FAPESP's process: 17/08123-5 - Bioproduction of antimicrobials, proteases and symbiotic products of food industry concern
Grantee:Ricardo Pinheiro de Souza Oliveira
Support type: Research Grants - Visiting Researcher Grant - International
FAPESP's process: 13/08617-7 - Production of extracellular L-asparaginase: from bioprospecting to the engineering of an antileukemic biopharmaceutical
Grantee:Adalberto Pessoa Junior
Support type: Research Projects - Thematic Grants
FAPESP's process: 14/10456-4 - Development and characterization of poly(ethylene glycol) methyl ether-Block-Poly (D,L-Lactide) (PEG-PLA) Polymersomes for the release of recombinant L-asparaginase
Grantee:Alexsandra Conceição Apolinário
Support type: Scholarships in Brazil - Doctorate