Hydrogen Production by Clostridium cellulolyticum a Cellulolytic and Hydrogen-Producing Bacteria Using Sugarcane Bagasse

Author(s)

Braga, Juliana K. ^[1] ; Abreu, Angela A. ^[2] ; Motteran, Fabricio ^[1] ; Pereira, Maria Alcina ^[2] ; Alves, Maria Madalena ^[2] ; Varesche, Maria Bernadete A. ^[1]

Affiliation

^[1] Univ Sao Paulo, Sch Engn Sao Carlos, Dept Hydraul & Sanitat, Sao Carlos, SP - Brazil

^[2] Univ Minho, CEB, Campus Gualtar, Braga - Portugal

• Document type: Journal article
• Source: WASTE AND BIOMASS VALORIZATION; v. 10, n. 4, p. 827-837, APR 2019.
• Web of Science Citations: 0

Abstract

Hydrogen (H-2) production by Clostridium cellulolyticum was investigated. Anaerobic batch reactors were operated with cellobiose (2g/L) and pretreated sugarcane bagasse (SCB) (2g/L) using a hydrothermal system to observe the effects of carbon source on H-2 production. Salts (NH4Cl, NaCl, MgCl2 and CaCl2) and vitamins (biotin, nicotinamide, p-aminobenzoic acid, thiamine, pantothenic acid, pyridoxamine, cyanocobalamin, riboflavin, folic and lipoic acid) were supplemented from stock solutions at different volumes percentages, ranging from 0 to 5%. The optimal concentration was 2.5% and the strain used both substrates and produced H-2 which was higher for cellobiose (14.9 +/- 0.2mmol/L) than for SCB (7.6 +/- 0.2mmol/L), although the phase was much smaller when SCB (59.9h) was used in relation to the assay with cellobiose (164h). H-2 was produced from SCB primarily through the fermentation of lactic and acetic acids. (AU)

• FAPESP's process: 13/20196-7 - Hydrolysis of sugar cane bagasse for the hydrogen and biogas production with suspended and immobilized in nanocoatings biomass
• Grantee: Juliana Kawanishi Braga
• Support Opportunities: Scholarships in Brazil - Post-Doctoral