Hydrolysis of sugar cane bagasse for the hydrogen production in mesophilic condition
| Grant number: | 13/20196-7 |
| Support type: | Scholarships in Brazil - Post-Doctorate |
| Effective date (Start): | May 01, 2014 |
| Effective date (End): | February 28, 2018 |
| Field of knowledge: | Engineering - Sanitary Engineering |
| Cooperation agreement: | Coordination of Improvement of Higher Education Personnel (CAPES) |
| Principal Investigator: | Maria Bernadete Amâncio Varesche |
| Grantee: | Juliana Kawanishi Braga |
| Home Institution: | Escola de Engenharia de São Carlos (EESC). Universidade de São Paulo (USP). São Carlos , SP, Brazil |
| Associated research grant: | 09/15984-0 - Bioenergy production from wastewaters and environmental fitting of liquid and solid wastes generated, AP.TEM |
| Associated scholarship(s): | 14/11851-4 - Hydrolysis of sugar cane bagasse for the hydrogen production in mesophilic condition, BE.EP.PD |
Abstract Lignocellulosic residues such as sugar cane bagasse are abundant sources of carbohydrates and their bioconversion has received great attention. It is known that the biological production of hydrogen and biogas makes sugar cane economically competitive since is low cost source of carbohydrates. Whereas the São Paulo state is the largest processor of sugarcane in Brazil and that this activity generates large amounts of waste rich in carbohydrates, especially sugar cane bagasse, this study will be evaluated its use as a substrate for the hydrogen and biogas (CH4 and CO2) production. For this project implementation we intend to conduct the bagasse hydrolysis using the fungus Aspergillus niger (stage 1), as well as the steam explosion. Tests in fermentative condition for H2 production (stage 2), and methanogenic-fermentative condition for biogas production (stage 3) will be performed with the hydrolyzed sugar cane bagasse and the residue from the end of the stage 2 respectively. The inocula for methanogenic-fermentative and fermentative tests will be from different origins, that is, in the first case fermentative bacteria enriched biomass will be used. In the second case inoculum from a mesophilic UASB reactor treating poultry DAKAR (Tiête - SP) will be applied. Synthetic substrate assay similar to stage 2 will be used in H2 production assay with immobilized bacteria in nanocoatings in a continuous flow reactor (stage 4). Finally microbial characterization will be performed by microbiological microscopy and molecular biology (PCR / DGGE of 16S rRNA sequencing and pyrosequencing). (AU) | |